LOCUS       sequence-73947-e        8150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  regulate protein stability with light.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ
  TITLE     A General Method for Regulating Protein Stability with Light.
  JOURNAL   ACS Chem Biol. 2014 Jan 17;9(1):111-5. doi: 10.1021/cb400755b
  PUBMED    24180414
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2014-01-17- 17"; volume: "9"; issue: "1"; 
            pages: "111-5"
COMMENT     Sequence Label: pBMN HAYFP-LOV24
FEATURES             Location/Qualifiers
     source          1..8150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1079..1495
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1425..1447
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1463..1479
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1505..1878
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1816..1832
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1896..1922
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1923..1925,1926..1928,1929..2639)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1968..1989)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2229..2248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2576..2597
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3120..3693,3694..3696,3697..3705)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3287..3304)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3514..3533
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3706..4389
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             4402..5088
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     LTR             5137..5730
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5861..5878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6032..6620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6112..6131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6794..7585,7586..7654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7417..7436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7722..7740
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaagc ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt
      901 agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccggg
     1081 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1141 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1201 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1261 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1321 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1381 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1441 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat
     1501 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga
     1561 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt
     1621 ccagcacgaa gtctggagac ctctggcggc acgtaccaag aacaactgga ccgaccggtg
     1681 gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac
     1741 ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa
     1801 gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt
     1861 ggaccatcct ctagactgcc ggatccgcca ccatgtatcc gtacgacgta ccggattatg
     1921 cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1981 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2101 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     2161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2521 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     2641 cgcgtttctt ggctactaca cttgaacgta ttgagaagaa ctttgtcatt actgacccaa
     2701 gattgccaga taatcccatt atattcgcgt ccgatagttt cttgcagttg acagaatata
     2761 gccgtgaaga aattttggga agaaactgca ggtttctaca aggtcctgaa actgatcgcg
     2821 cgacagtgag aaaaattaga gatgccatag ataaccaaac agaggtcact gttcagctga
     2881 ttaattatac aaagagtggt aaaaagttct ggaacctctt tcacttgcag cctatgcgag
     2941 atcagaaggg agatgtccag tactttattg gggttcagtt ggatggaact gagcatgtcc
     3001 gagatgctgc cgagagagag ggagtcatgc tggctaagaa aactgcagaa aatattgatg
     3061 aggcggcaag gcggcgaggt tagatgcatc gatccgcggc ggccgctacg taaattccgc
     3121 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3181 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     3241 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     3301 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     3361 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     3421 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     3481 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     3541 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     3601 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
     3661 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcggcctg
     3721 ctgaaggaga gtatgcgcat caagatgtac atggagggca ccgtgaacgg ccactacttc
     3781 aagtgcgagg gcgagggcga cggcaacccc ttcgccggca cccagagcat gagaatccac
     3841 gtgaccgagg gcgcccccct gcccttcgcc ttcgacatcc tggccccctg ctgcgagtac
     3901 ggcagcagga ccttcgtgca ccacaccgcc gagatccccg acttcttcaa gcagagcttc
     3961 cccgagggct tcacctggga gagaaccacc acctacgagg acggcggcat cctgaccgcc
     4021 caccaggaca ccagcctgga gggcaactgc ctgatctaca aggtgaaggt gcacggcacc
     4081 aacttccccg ccgacggccc cgtgatgaag aacaagagcg gcggctggga gcccagcacc
     4141 gaggtggtgt accccgagaa cggcgtgctg tgcggccgga acgtgatggc cctgaaggtg
     4201 ggcgaccggc acctgatctg ccaccactac accagctacc ggagcaagaa ggccgtgcgc
     4261 gccctgacca tgcccggctt ccacttcacc gacatccggc tccagatgct gcggaagaag
     4321 aaggacgagt acttcgagct gtacgaggcc agcgtggccc ggtacagcga cctgcccgag
     4381 aaggccaaca gatctcccgg gatggtgagc ggcctgctga aggagagtat gcgcatcaag
     4441 atgtacatgg agggcaccgt gaacggccac tacttcaagt gcgagggcga gggcgacggc
     4501 aaccccttcg ccggcaccca gagcatgaga atccacgtga ccgagggcgc ccccctgccc
     4561 ttcgccttcg acatcctggc cccctgctgc gagtacggca gcaggacctt cgtgcaccac
     4621 accgccgaga tccccgactt cttcaagcag agcttccccg agggcttcac ctgggagaga
     4681 accaccacct acgaggacgg cggcatcctg accgcccacc aggacaccag cctggagggc
     4741 aactgcctga tctacaaggt gaaggtgcac ggcaccaact tccccgccga cggccccgtg
     4801 atgaagaaca agagcggcgg ctgggagccc agcaccgagg tggtgtaccc cgagaacggc
     4861 gtgctgtgcg gccggaacgt gatggccctg aaggtgggcg accggcacct gatctgccac
     4921 cactacacca gctaccggag caagaaggcc gtgcgcgccc tgaccatgcc cggcttccac
     4981 ttcaccgaca tccggctcca gatgctgcgg aagaagaagg acgagtactt cgagctgtac
     5041 gaggccagcg tggcccggta cagcgacctg cccgagaagg ccaactgagt cgacgataaa
     5101 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     5161 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     5221 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
     5281 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
     5341 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     5401 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
     5461 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     5521 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     5581 tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     5641 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     5701 ttgactaccc gtcagcgggg gtctttcatt cacatgcagc atgtatcaaa attaatttgg
     5761 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
     5821 gcggggagag gcggtttgcg tattggcgct cttccgcttc ctcgctcact gactcgctgc
     5881 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5941 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6661 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6721 agatcctttt aaattaaaaa tgaagtttgc gcaaatcaat ctaaagtata tatgagtaaa
     6781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7081 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7381 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     7441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7681 gcatttatca gggttattgt ctcatgacat taacctataa aaataggcgt atcacgaggc
     7741 cctttcgtct tcaagaacag ctttgctctt aggagtttcc taatacatcc caaactcaaa
     7801 tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta agccagcttt
     7861 ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat ttcataaggg
     7921 tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa ataaaaatag
     7981 ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca gttgacaaca
     8041 taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca ttatgccagt
     8101 catattaatt actagtcaat tagttgattt ttatttttga catatacatg
//
