LOCUS       sequence-73949-e        7912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab6BQ72LdeltaCSC in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab6BQ72LdeltaCSC
FEATURES             Location/Qualifiers
     source          1..7912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      1946..1993
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2020..2207
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2075..2099)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2231..2247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2231..2247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2244..2266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2255..2271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2279..2285,2286..2303,2304..2309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(2462..2479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2633..3221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2713..2732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3550..3597
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3622..3641
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3830..4624)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3941..3960)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4551..4570
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4625..4729)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4756..6098
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5719..5766)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6139..6157
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6195..6217)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6317..6336
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6357..6638
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6639..7313
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7416..7871)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7553..7574)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7765..7784
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcatgtcc gcagggggag
     1321 attttgggaa tccactgaga aaattcaagt tggtgttctt gggggagcag agcgtcggga
     1381 agacgtctct gattacgagg ttcatgtacg acagcttcga caacacatac caggcaacca
     1441 ttgggattga cttcttgtca aaaaccatgt acttggagga ccgcacggtg cgactgcagc
     1501 tctgggacac agctggtctg gagaggttcc gcagcctgat ccccagctac atccgggact
     1561 ccacggtggc tgtggtggtg tacgacatca caaatctcaa ctccttccaa cagacctcta
     1621 agtggatcga cgacgtcagg acagagaggg gcagtgatgt tatcatcatg ctggtgggca
     1681 acaagacgga cctggctgat aagaggcaga taaccatcga ggagggggag cagcgcgcca
     1741 aagaactgag cgtcatgttc attgagacca gtgcgaagac tggctacaac gtgaagcagc
     1801 tttttcgacg tgtggcgtcg gctctacccg gaatggagaa tgtccaggag aaaagcaaag
     1861 aagggatgat cgacatcaag ctggacaaac cccaggagcc cccggccagc gagggcggct
     1921 aactcgacct gcagcggccg cataactagc ataacccctt ggggcctcta aacgggtctt
     1981 gaggggtttt ttgcgcgctt gcagccaagc taattccggg cgaatttctt atgatttatg
     2041 atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct
     2101 taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt
     2161 tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat gcaagcttgg
     2221 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2281 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca
     2341 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2401 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3061 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3301 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3361 aaagtatata tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga
     3421 gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt
     3481 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc
     3541 cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca
     3601 tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt
     3661 gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga
     3721 aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg
     3781 gcaggttggg cgtcgcttgg tcggttcatt tcgaacccca gagtcccgct cagaagaact
     3841 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     3901 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     3961 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     4021 ggccattttc caccatgata ttcggcaagc aggcatcgtc atgggtcacg acgagatcct
     4081 cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     4141 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     4201 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     4261 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     4321 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     4381 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     4441 cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     4501 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     4561 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     4621 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4681 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4741 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     4801 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     4861 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
     4921 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     4981 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
     5041 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
     5101 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
     5161 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
     5221 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
     5281 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
     5341 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
     5401 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
     5461 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
     5521 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
     5581 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
     5641 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
     5701 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
     5761 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
     5821 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
     5881 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
     5941 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
     6001 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
     6061 tcatccttca atgctatcat ttcctttgat attggatcat attaagaaac cattattatc
     6121 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     6181 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6241 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6301 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tagatcaacg
     6361 acattactat atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta
     6421 ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt
     6481 gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt
     6541 cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt
     6601 gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag
     6661 gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg
     6721 ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa
     6781 ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag
     6841 gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc
     6901 tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat
     6961 accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca
     7021 tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag
     7081 caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag
     7141 agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg
     7201 atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg
     7261 gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta
     7321 ctgagtagta tttatttaag tattgtttgt gcacttgccg atctatgcgg tgtgaaatac
     7381 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta atattttgtt
     7441 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7501 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7561 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7621 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7681 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7741 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7801 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7861 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg at
//
