LOCUS       sequence-73964-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(518..542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             895..936
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctc
      121 atatgagcgc aggaaaacag taggaaagtg acttcaaaat ggaaaatttg acattcagtg
      181 actgaagcat tggtatgata atttctaaca cttgtagaag gtccatgact gtaattttac
      241 caatgaaaag catttaactg ttttggattc caaactagca gcactacaat gctttgctag
      301 agctggtaaa atggaaccaa atcgcctctt caatggattt ggtccccttc aaccagctgt
      361 agctatgcat tgattactac gggacaacca acgttttcat ttgtgaatat caattacttg
      421 ccaactaatt tcaacttatt tacattatgc agactgtgat tataatcaaa taacttgtta
      481 ctgttaattc tacctggaaa agctagaatg gaagcttacc cagctttctt gtacaaagtg
      541 gttgatctag agggcccgca gtggggtcag cttctaccgg ggcggcgtcc cggggtcttg
      601 gaactgcatg cagactgcct gcttgggaaa catacttctt tatatgccca tatggacctg
      661 ctaagctatg gaatgtaaag aagtatgtat ctcaggccgg gacctctctc gccgcactga
      721 ggggcactcc acaccacggg ggccgccgcg gccgacagcc agccaggcgg ctgtggtggg
      781 ggtgaggctg gggcggcagg ctgcctgggc gttggtggac ggtcgtgtcc ccgccagcat
      841 tgttctgtca ctttgcctgt gtcagcaggt ggaagtgctc accagacatt cgaaggtaag
      901 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagtttaa
      961 acgggg
//
