LOCUS       sequence-73968-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-5
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(302..326)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             679..720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(760..779)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    804..852
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 ctctattttt actaattctg aattccataa ttctatattc tgtattcctc cccgccggcc
      181 gctgatatgt ctggtattct tgggtttgaa cttccagcgt tgaacccgca tattagacgt
      241 atcgacggcc ggcggggcat aatctgctaa gtttggagag accgggggag tcccagagat
      301 gccccagctt tcttgtacaa agtggttgat ctagagggcc cgcagtgggg tcagcttcta
      361 ccggggcggc gtcccggggt cttggaactg catgcagact gcctgcttgg gaaacatact
      421 tctttatatg cccatatgga cctgctaagc tatggaatgt aaagaagtat gtatctcagg
      481 ccgggacctc tctcgccgca ctgaggggca ctccacacca cgggggccgc cgcggccgac
      541 agccagccag gcggctgtgg tgggggtgag gctggggcgg caggctgcct gggcgttggt
      601 ggacggtcgt gtccccgcca gcattgttct gtcactttgc ctgtgtcagc aggtggaagt
      661 gctcaccaga cattcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
      721 cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat
      781 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
      841 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
      901 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccc
//
