LOCUS       sequence-73969-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir-122
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(369..380)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(525..549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             902..943
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagttaag ctatcaacaa gtttgtacaa aaaagcaggc
      121 tcatatggtt cagctcttcc cattgctcaa gatgcttgta cccgtgatgc ttcttttctc
      181 tgcttaggtc acaatatgtg gagctgacaa ggttccccta ttatcagtga caatggtgga
      241 atgtggaggt gaagttaaca ccttcgtggc tacagagttt ccttagcaga gctgtggagt
      301 gtgacaatgg tgtttgtgtc taaactatca aacgccatta tcacactaaa tagctactgc
      361 taggcaatcc ttccctcgat aaatgtcttg gcatcgtttg ctttgagcaa gaaggttcat
      421 ctgatatcag tcttctcaat cttgtgtact tactgaataa agtctggttc ttttgcactc
      481 ataaccaata tggttagctg aaccctgcct ctcacatgaa gcttacccag ctttcttgta
      541 caaagtggtt gatctagagg gcccgcagtg gggtcagctt ctaccggggc ggcgtcccgg
      601 ggtcttggaa ctgcatgcag actgcctgct tgggaaacat acttctttat atgcccatat
      661 ggacctgcta agctatggaa tgtaaagaag tatgtatctc aggccgggac ctctctcgcc
      721 gcactgaggg gcactccaca ccacgggggc cgccgcggcc gacagccagc caggcggctg
      781 tggtgggggt gaggctgggg cggcaggctg cctgggcgtt ggtggacggt cgtgtccccg
      841 ccagcattgt tctgtcactt tgcctgtgtc agcaggtgga agtgctcacc agacattcga
      901 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtta
//
