LOCUS       sequence-73971-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir40-5
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(243..267)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             620..661
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(701..720)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    745..793
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa
       61 gtttgtacaa aaaagcaggc tggtgtaaat cagcttggct ctggtgtctc cgaaccttgt
      121 gtccgcacct cagtggatgt atgccatgat gataagatat cagaaatcct atcaccgggt
      181 gtacatcagc taaggtgcgg gtacacatgc cttcgatatc aaggtgagtt atttttaagt
      241 gggcccagct ttcttgtaca aagtggttga tctagagggc ccgcagtggg gtcagcttct
      301 accggggcgg cgtcccgggg tcttggaact gcatgcagac tgcctgcttg ggaaacatac
      361 ttctttatat gcccatatgg acctgctaag ctatggaatg taaagaagta tgtatctcag
      421 gccgggacct ctctcgccgc actgaggggc actccacacc acgggggccg ccgcggccga
      481 cagccagcca ggcggctgtg gtgggggtga ggctggggcg gcaggctgcc tgggcgttgg
      541 tggacggtcg tgtccccgcc agcattgttc tgtcactttg cctgtgtcag caggtggaag
      601 tgctcaccag acattcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      661 gcgtaccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa
      721 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
      781 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
      841 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccc
//
