LOCUS       sequence-73973-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir40-4
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(276..300)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             653..694
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(734..753)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    778..826
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctggtgta
      121 aatcagcttg gctctggtgt ctccgaacct tgtgtccgca cctcagtgga tgtatgccat
      181 gatgataaga tatcagaaat cctatcaccg ggtgtacatc agctaaggtg cgggtacaca
      241 tgcatctgat atcaaggtga gttattttta agtgggccca gctttcttgt acaaagtggt
      301 tgatctagag ggcccgcagt ggggtcagct tctaccgggg cggcgtcccg gggtcttgga
      361 actgcatgca gactgcctgc ttgggaaaca tacttcttta tatgcccata tggacctgct
      421 aagctatgga atgtaaagaa gtatgtatct caggccggga cctctctcgc cgcactgagg
      481 ggcactccac accacggggg ccgccgcggc cgacagccag ccaggcggct gtggtggggg
      541 tgaggctggg gcggcaggct gcctgggcgt tggtggacgg tcgtgtcccc gccagcattg
      601 ttctgtcact ttgcctgtgt cagcaggtgg aagtgctcac cagacattcg aaggtaagcc
      661 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttaaac
      721 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
      781 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
      841 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
      901 cgcgtttctt ccttttcccc accccacccc ccaagttc
//
