LOCUS       sequence-73974-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir30-1
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           192..206
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(284..308)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             661..702
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(742..761)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    786..834
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctggttaa
      121 cccaacagaa ggctaaagaa ggtatattgc tgttgccagt gagcgactgt aaacatcctc
      181 gactggaagc tgtgaagcca cagatgggct ttcagtcgga tgtttgcagc tgcctactgg
      241 ctcggacttc aaggggctac tttaggagca attatcttgt ttaccccagc tttcttgtac
      301 aaagtggttg atctagaggg cccgcagtgg ggtcagcttc taccggggcg gcgtcccggg
      361 gtcttggaac tgcatgcaga ctgcctgctt gggaaacata cttctttata tgcccatatg
      421 gacctgctaa gctatggaat gtaaagaagt atgtatctca ggccgggacc tctctcgccg
      481 cactgagggg cactccacac cacgggggcc gccgcggccg acagccagcc aggcggctgt
      541 ggtgggggtg aggctggggc ggcaggctgc ctgggcgttg gtggacggtc gtgtccccgc
      601 cagcattgtt ctgtcacttt gcctgtgtca gcaggtggaa gtgctcacca gacattcgaa
      661 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
      721 gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      781 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
      841 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
      901 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaa
//
