LOCUS       sequence-73975-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir30-2
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           203..217
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(295..319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             672..713
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(753..772)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    797..845
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctggtta acccaacaga aggctaaaga aggtatattg ctgtagacag tgagcgactg
      181 taaacatcct cgactggaag ctgtgaagcc acagatgggc tttcagtcgg atgtttgcag
      241 ctgcctactg cctcggactt caaggggcta ctttaggagc aattatcttg tttaccccag
      301 ctttcttgta caaagtggtt gatctagagg gcccgcagtg gggtcagctt ctaccggggc
      361 ggcgtcccgg ggtcttggaa ctgcatgcag actgcctgct tgggaaacat acttctttat
      421 atgcccatat ggacctgcta agctatggaa tgtaaagaag tatgtatctc aggccgggac
      481 ctctctcgcc gcactgaggg gcactccaca ccacgggggc cgccgcggcc gacagccagc
      541 caggcggctg tggtgggggt gaggctgggg cggcaggctg cctgggcgtt ggtggacggt
      601 cgtgtccccg ccagcattgt tctgtcactt tgcctgtgtc agcaggtgga agtgctcacc
      661 agacattcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
      721 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
      781 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
      841 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
      901 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttc
//
