LOCUS       sequence-73976-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir30-wt
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           200..214
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(292..316)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             669..710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(750..769)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    794..842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 ctggttaacc caacagaagg ctaaagaagg tatattgctg ttgacagtga gcgactgtaa
      181 acatcctcga ctggaagctg tgaagccaca gatgggcttt cagtcggatg tttgcagctg
      241 cctactgcct cggacttcaa ggggctactt taggagcaat tatcttgttt accccagctt
      301 tcttgtacaa agtggttgat ctagagggcc cgcagtgggg tcagcttcta ccggggcggc
      361 gtcccggggt cttggaactg catgcagact gcctgcttgg gaaacatact tctttatatg
      421 cccatatgga cctgctaagc tatggaatgt aaagaagtat gtatctcagg ccgggacctc
      481 tctcgccgca ctgaggggca ctccacacca cgggggccgc cgcggccgac agccagccag
      541 gcggctgtgg tgggggtgag gctggggcgg caggctgcct gggcgttggt ggacggtcgt
      601 gtccccgcca gcattgttct gtcactttgc ctgtgtcagc aggtggaagt gctcaccaga
      661 cattcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt
      721 agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
      781 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
      841 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
      901 ttgggg
//
