LOCUS       sequence-73977-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir-142
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(535..559)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 ctcatatggg gaggtagagg aggcaagtct ggcgccatgc tgagtcaccg cccacaaggc
      181 ccagggcggg ccctcggggg gccctggcag ggttgggggg atcttaggaa gccacaagga
      241 gggctggggg gctcttggag caggagtcag gaggcctggg cagcctgaag agtacacgcc
      301 gacggacaga cagacagtgc agtcacccat aaagtagaaa gcactactaa cagcactgga
      361 gggtgtagtg tttcctactt tatggatgag tgtactgtgg gcttcggaga tcacgccact
      421 gctgccgccc gctgcccgcc accatcttcc tcggcgctcg gggacctcgt gtgacaggtg
      481 agcaccttac ggcccctccc taccctgccc agatgcctga aaggcctcaa gcttacccag
      541 ctttcttgta caaagtggtt gatctagagg gcccgcagtg gggtcagctt ctaccggggc
      601 ggcgtcccgg ggtcttggaa ctgcatgcag actgcctgct tgggaaacat acttctttat
      661 atgcccatat ggacctgcta agctatggaa tgtaaagaag tatgtatctc aggccgggac
      721 ctctctcgcc gcactgaggg gcactccaca ccacgggggc cgccgcggcc gacagccagc
      781 caggcggctg tggtgggggt gaggctgggg cggcaggctg cctgggcgtt ggtggacggt
      841 cgtgtccccg ccagcattgt tctgtcactt tgcctgtgtc agcaggtgga agtgctcacc
      901 agacattc
//
