LOCUS       sequence-73978-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning mammalian cell line expression vector with CMV promoter 
            and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C 
            protease cleavage site .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pcDXNSMG3
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..42
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(91..396)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(193..212)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gagctgtaca agggtacctt agaagttttg tttcaaggtc caagtagaag agcgagctgc
       61 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
      121 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
      181 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
      241 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
      301 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
      361 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
      421 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
      481 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
      541 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
      601 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
      661 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt
      721 aagccggatc cacgcgttta cgccccgccc tgccactcat cgcagtactg ttgtaattca
      781 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
      841 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
      901 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccc
//
