LOCUS       sequence-73979-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian 
            cells. cDNA is synthetic, containing convenient restriction sites. 
            Native hNKCC1 amino acid sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             116..202
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDY"
     primer_bind     complement(257..278)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(518..537)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     865..886
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cccgggcacg gccgccacca tgggataccc
      121 ttacgatgtc cctgactacg ccggctaccc atacgacgtg ccagactacg ctgggtccta
      181 cccctacgac gttcccgatt acggtgaccc taccgtcagc aagggcgagg agctgttcac
      241 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttctcggt
      301 ctctggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      361 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      421 gtgcttcgct cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      481 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      541 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      601 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
      661 cgtttatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      721 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      781 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca aactgagcaa
      841 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      901 cactctcggc atggacga
//
