LOCUS       sequence-7404-ex        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Sharma DK, Brown JC, Choudhury A, Peterson TE, Holicky E, Marks DL, 
            Simari R, Parton RG, Pagano RE
  TITLE     Selective stimulation of caveolar endocytosis by glycosphingolipids 
            and cholesterol.
  JOURNAL   Mol Biol Cell. 2004 Jul . 15(7):3114-22.
  PUBMED    15107466
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2004 Jul . 15(7):3114-22."
COMMENT     Sequence Label: Cav1-mRed
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             596..1273
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             596..1273
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(726..744)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     994..1013
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1188..1211
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aagcttcgaa ttcggcacga gcttcaccca gccaccggcc agcatgtctg ggggcaaata
       61 cgtagactcc gaggggcacc tctacaccgt tcccatccgg gagcagggca acatctacaa
      121 gcccaacaac aaggccatgg cggaggagat gagcgagaag caggtgtacg acgcgcacac
      181 caaggaaatc gacctggtca accgcgaccc caagcatctc aacgacgacg tggtcaagat
      241 tgattttgaa gatgtgattg cagaaccaga aggaacacac agttttgatg gcatctggaa
      301 ggccagcttc accaccttca ctgtgacaaa atactggttt taccgcttgc tgtctgccct
      361 ctttggcatc ccaatggcac tcatatgggg catttacttt gccattcttt ctttcctgca
      421 catctgggca gttgtgccgt gcattaagag tttcctgatt gagattcagt gcatcagccg
      481 tgtctattcc atctacgtcc acaccttctg tgacccgttc tttgaggctg ttggcaaaat
      541 attcagcaat atccgcatca acatgcagaa agaaacaccg cgggcccggg atccgatggc
      601 ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt
      661 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      721 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
      781 ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      841 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      901 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      961 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     1021 ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     1081 gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat
     1141 ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac
     1201 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc
     1261 caccggcgcc taagaattct gcagatatc
//
