LOCUS       sequence-7412-ex        2993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB
  TITLE     Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors 
            in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing 
            Mutants.
  JOURNAL   Plant Cell. 2006 Sep . 18(9):2314-25.
  PUBMED    16935985
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2006 Sep . 18(9):2314-25."
COMMENT     Sequence Label: pFLIPglu-600uDelta13
FEATURES             Location/Qualifiers
     source          1..2993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        96..441
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     385..405
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(453..455,456..458,459..971)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2505..2756
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gaatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga acatcgtgga
      301 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      361 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      421 ttcatttcat ttggagagga cctcgagggc ccatggtgag caagggcgag gagctgttca
      481 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      541 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      601 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      661 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      721 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      781 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      841 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca
      901 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
      961 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1021 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1081 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1141 tcgctgatac tcgcattggt gtaacaatct ataagtacga cgataacgcg atgtctgtag
     1201 tgcgcaaggc tattgagcaa gatgcgaaag ccgcgccaga tgttcagctg ctgatgaatg
     1261 attctcagaa tgaccagtcc aagcagaacg atcagatcga cgtattgctg gcgaaagggg
     1321 tgaaggcact ggcaatcaac ctggttgacc cggcagctgc gggtacggtg attgagaaag
     1381 cgcgtgggca aaacgtgccg gtggttttct tcaacaaaga accgtctcgt aaggcgctgg
     1441 atagctacga caaagcctac tacgttggca ctgactccaa agagtccggc attattcaag
     1501 gcgatttgat tgctaaacac tgggcggcga atcagggttg ggatctgaac aaagacggtc
     1561 agattcagtt cgtactgctg aaaggtgaac cgggccatcc ggatgcagaa gcacgtacca
     1621 cttacgtgat taaagaattg aacgataaag gcatcaaaac tgaacagtta cagttagata
     1681 ccgcaatgtg ggacaccgct caggcgaaag ataagatgga cgcctggctg tctggcccga
     1741 acgccaacaa aatcgaagtg gttatcgcca acaacgatgc gatggcaatg ggcgcggttg
     1801 aagcgctgaa agcacacaac aagtccagca ttccggtgtt tggcgtcgat gcgctgccag
     1861 aagcgctggc gctggtgaaa tccggtgcac tggcgggcac cgtactgaac gatgctaaca
     1921 accaggcgaa agcgaccttt gatctggcga aaaacctggc cgatggtaaa ggtgcggctg
     1981 atggcaccaa ctggaaaatc gacaacaaag tggtccgcgt accttatgtt ggcgtagata
     2041 aagacaacct ggctctcgag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2101 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2161 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2221 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     2281 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2341 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2401 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2461 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2521 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2581 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2641 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2701 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     2761 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct attttctcca
     2821 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     2881 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     2941 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctt
//
