LOCUS       sequence-7413-ex        2990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB
  TITLE     Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors 
            in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing 
            Mutants.
  JOURNAL   Plant Cell. 2006 Sep . 18(9):2314-25.
  PUBMED    16935985
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2006 Sep . 18(9):2314-25."
COMMENT     Sequence Label: pFLIPglu-3.2mDelta13
FEATURES             Location/Qualifiers
     source          1..2990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        95..440
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     384..404
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(452..454,455..457,458..970)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2504..2755
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
ORIGIN
        1 agcttgcatg cctgcaggtc aacatggtgg agcacgacac tctcgtctac tccaagaata
       61 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      121 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      181 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
      241 aatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      361 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      421 tcatttcatt tggagaggac ctcgagggcc catggtgagc aagggcgagg agctgttcac
      481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      721 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      841 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
      901 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      961 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1021 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1081 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1141 cgctgatact cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtagt
     1201 gcgcaaggct attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga
     1261 ttctcagaat gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt
     1321 gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc
     1381 gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa ccgtctcgta aggcgctgga
     1441 tagctacgac aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaagg
     1501 cgatttgatt gctaaacact gggcggcgaa tcagggttgg gatctgaaca aagacggtca
     1561 gattcagttc gtactgctga aaggtgaacc gggcgctccg gatgcagaag cacgtaccac
     1621 ttacgtgatt aaagaattga acgataaagg catcaaaact gaacagttac agttagatac
     1681 cgcaatgtgg gacaccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa
     1741 cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggttga
     1801 agcgctgaaa gcacacaaca agtccagcat tccggtgttt ggcgtcgatg cgctgccaga
     1861 agcgctggcg ctggtgaaat ccggtgcact ggcgggcacc gtactgaacg atgctaacaa
     1921 ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc gatggtaaag gtgcggctga
     1981 tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa
     2041 agacaacctg gctctcgagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2101 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2161 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2221 cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
     2281 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2341 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2401 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2461 gctggagtac aactacaaca gccacaacgt ttatatcatg gccgacaagc agaagaacgg
     2521 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2581 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2641 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2701 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaatc
     2761 tagagtccgc aaatcaccag tctctctcta caaatctatc tctctctatt ttctccagaa
     2821 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     2881 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     2941 atttctaatt cctaaaacca aaatccagtg acctgcaggc atgcaagctt
//
