LOCUS       sequence-74190-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic G11-5FLAG epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-G11-5FLAG-kanMX6
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..211
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     terminator      238..425
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(293..317)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        500..843
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(911..930)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 agcttcgtac gctgcaggtc gacggatccc cgggggaggt ggaggtggag gtggaggtgg
       61 aggtttaatt aatcatatgg actacaagga cgacgatgac aaggattaca aagatgacga
      121 cgataagctt atggactaca aggacgacga tgacaaggat tacaaagatg acgacgataa
      181 gcttatggac tacaaggacg acgatgacaa gcatatgggc gcgccacttc taaataagcg
      241 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
      301 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
      361 ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct
      421 accggcagat ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc
      481 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
      541 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
      601 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
      661 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
      721 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
      781 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
      841 accatgggta aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct
      901 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      961 cgattgtatg ggaagcccga tgcgccagag ttg
//
