LOCUS       sequence-74192-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant lacking cysteine residues and with an 
            N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, 
            containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtccatgtg tacgtggcca gagatcatca aaccaacatt ttttgtgaaa tcgtgaacaa
       61 gatgaagtaa ggccggccgt gagtttggcg ctcctgtcat aacaagggac tgtggcctaa
      121 agtttttcac atggtcttcc acgccagaaa gcctaatact atgttgcagg gcattgaggt
      181 aagtcagggc ttgtgtagag gatccccaat tcacatcggg ttttttgtag gtaacataaa
      241 tatagagacc aaggactatc acgtaggtta gcaaagcagc ccaccagtta atgacgaaca
      301 ttactatcac ggaaagaatt gctccaagaa gtgagatcca catattgtag tatttaaatg
      361 ccggtcgcca tcctgggctt tttgccagtg aagcatggaa tactgaaaaa ttgatgaggg
      421 catagctggc aaggaagaag tttgagataa ttggtgcaat aacattcagt tcggcaatta
      481 agatgaatcc aagtgcaatt aagaatgtta agatgtagcc acgtaatggc tcattatttt
      541 tcccataacc tttagcaaac atctggaaag cggggtagat gttgtctttg gatagggcct
      601 gaaatatctt gggagcactc actagggatg ctaatgcgga agacagagtg gcagagaata
      661 tgcccgcgga aattagtggt gtaaatcctg agaccatact cattacctga aagttgttca
      721 ttaggccgta gctagaagga ctggattcag aagatgaaaa atcaaagttt aatttagagg
      781 ctgcgctagt agagtttgtt agctctgtta cgatagtgtc attaacatta ccagtggcat
      841 cgcgtacgac catagaacct acagacacgg caattcctac ataaaccaat gtagtaatta
      901 aaatggctag gagtgttcct ttgggtatgg ctgactgag
//
