LOCUS       sequence-74193-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant lacking cysteine residues and with an 
            N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, 
            containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acattccatt cttatgatag atgaaatcaa tgacatccgg attattggag ccattacagt
       61 cgtgattctt ttaggtatct cagtagccgg tatggagtgg gaagcaaaag ctcagattgt
      121 tcttttggtg atcctacttc ttgctattgg tgatttcgtc ataggaacat ttatcccact
      181 cgagagcaag aagccaaaag ggttttttgg ctataaatct gaaatcttta atgagaactt
      241 tggccccgat tttcgagaag aagaaacttt cttttctgta tttgccatct tttttcctgc
      301 tgcaactgga attctggctg gcgcaaatat ctcaggtgat cttgcagatc ctcagtcagc
      361 catacccaaa ggaacactcc tagccatttt aattactaca ttggtttatg taggaattgc
      421 cgtgtctgta ggttctatgg tcgtacgcga tgccactggt aatgttaatg acactatcgt
      481 aacagagcta acaaactcta ctagcgcagc ctctaaatta aactttgatt tttcatcttc
      541 tgaatccagt ccttctagct acggcctaat gaacaacttt caggtaatga gtatggtctc
      601 aggatttaca ccactaattt ccgcgggcat attctctgcc actctgtctt ccgcattagc
      661 atccctagtg agtgctccca agatatttca ggccctatcc aaagacaaca tctaccccgc
      721 tttccagatg tttgctaaag gttatgggaa aaataatgag ccattacgtg gctacatctt
      781 aacattctta attgcacttg gattcatctt aattgccgaa ctgaatgtta ttgcaccaat
      841 tatctcaaac ttcttccttg ccagctatgc cctcatcaat ttttcagtat tccatgcttc
      901 actgg
//
