LOCUS       sequence-74197-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtgtacgtg tccacagatc atcaaaccaa cattttttgt gaaatcgtga acaagatgaa
       61 gtaaggccgg ccgtgagttt ggcgctcctg tcataacaag acactgtggc ctaaagtttt
      121 tcacatggtc ctccacgcca gaaagcctaa tactatgttg cagggcattg aggtaagtca
      181 gggcttgtgt agaggatccc caattcacat cgggtttttt gtaggtaaca taaatataca
      241 gcccaaggac tatcacgtag gttagcaaag cagcccacca gttaatgacg aacattacta
      301 tgcaacaaag aattgctcca agaagtgaga tccacatatt gtagtattta aatgccggtc
      361 gccatcctgg gctttttgcc agtgaagcat ggaatactga aaaattgatg agggcatagc
      421 tggcaaggaa gaagtttgag ataattggtg caataacatt cagttcggca attaagatga
      481 atccaagtgc aattaagaat gttaagatgt agccacgtaa tggctcatta tttttcccat
      541 aacctttagc aaacatctgg aaagcggggt agatgttgtc tttggctaga gcctggaata
      601 tcttgggagc actcactagg gatgctaatg cggaagacag agtggcagag aatatgcccg
      661 cggaaattag tggtgtaaat cctgacacca tactcattac ctgaaagttg ttcattaggc
      721 cgtagctaca aggactggat tcacaagatg aaaaatcaaa gtttaatttg caggctgcgc
      781 tagtacagtt tgttagctct gttacgatag tgtcattaac attaccagtg gcatcgcgta
      841 cgacacaaga acctacagac acggcaattc ctacataaac caatgtagta attaaaatgg
//
