LOCUS       sequence-74203-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 C543M (NT360)
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acattccatt cttatgatag atgaaatcaa tgacatccgg attattggag ccattacagt
       61 cgtgattctt ttaggtatct cagtagccgg tatggagtgg gaagcaaaag ctcagattgt
      121 tcttttggtg atcctacttc ttgctattgg tgatttcgtc ataggaacat ttatcccact
      181 cgagagcaag aagccaaaag ggttttttgg ctataaatct gaaatcttta atgagaactt
      241 tggccccgat tttcgagaag aagaaacttt cttttctgta tttgccatct tttttcctgc
      301 tgcaactgga attctggctg gcgcaaatat ctcaggtgat cttgcagatc ctcagtcagc
      361 catacccaaa ggaacactcc tagccatttt aattactaca ttggtttatg taggaattgc
      421 cgtgtctgta ggttctatgg tcgtacgcga tgccactggt aatgttaatg acactatcgt
      481 aacagagcta acaaactgta ctagcgcagc ctgcaaatta aactttgatt tttcatcttg
      541 tgaatccagt ccttgtagct acggcctaat gaacaacttt caggtaatga gtatggtgtc
      601 aggatttaca ccactaattt ccgcgggcat attctctgcc actctttcta gcgcattagc
      661 atccctagtg agtgctccca agatatttca ggccctatgt aaagacaaca tctaccccgc
      721 tttccagatg tttgctaaag gttatgggaa aaataatgag ccattacgtg gctacatctt
      781 aacattctta attgcacttg gattcatctt aattgccgaa ctgaatgtta ttgcaccaat
      841 tatctcaaac ttcttccttg ccagctatgc cctcatcaat ttttcagtat tccatgcttc
      901 actggcaaaa agcccaggat ggcgaccggc atttaaatac tacaatatgt gg
//
