LOCUS       sequence-74206-e         527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pAGM1287
FEATURES             Location/Qualifiers
     source          1..527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..109)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(251..269)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        337..441
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
       61 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
      121 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      181 tccgcttaca gacaagctgt gacgaagaca attcgtgaga ccacgaagtg gctcttcagt
      241 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      301 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      361 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      421 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      481 agcggtggta acggcgcaat ggcggttttc atggcttgtt atgactg
//
