LOCUS       sequence-74208-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant with HA tag inserted into extracellular
            loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is 
            synthetic, containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             188..214
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             218..244
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 acattccatt cttatgatag atgaaatcaa tgacatccgg attattggag ccattacagt
       61 cgtgattctt ttaggtatct cagtagccgg tatggagtgg gaagcaaaag ctcagattgt
      121 tcttttggtg atcctacttc ttgctattgg tgatttcgtc ataggaacat ttatcccact
      181 cgagagctac ccttacgatg tccctgacta cgccggctac ccatacgacg tgccagacta
      241 cgctaagaag ccaaaagggt tttttggcta taaatctgaa atctttaatg agaactttgg
      301 ccccgatttt cgagaagaag aaactttctt ttctgtattt gccatctttt ttcctgctgc
      361 aactggaatt ctggctggcg caaatatctc aggtgatctt gcagatcctc agtcagccat
      421 acccaaagga acactcctag ccattttaat tactacattg gtttatgtag gaattgccgt
      481 gtctgtaggt tcttgtgtcg tacgcgatgc cactggtaat gttaatgaca ctatcgtaac
      541 agagctaaca aactgtacta gcgcagcctg caaattaaac tttgattttt catcttgtga
      601 atccagtcct tgtagctacg gcctaatgaa caactttcag gtaatgagta tggtgtcagg
      661 atttacacca ctaatttccg cgggcatatt ctctgccact ctttctagcg cattagcatc
      721 cctagtgagt gctcccaaga tatttcaggc cctatgtaaa gacaacatct accccgcttt
      781 ccagatgttt gctaaaggtt atgggaaaaa taatgagcca ttacgtggct acatcttaac
      841 attcttaatt gcacttggat tcatcttaat tgccgaactg aatgttattg caccaattat
      901 ctcaaacttc ttccttgcca gctatgccct catcaatttt tcagtattcc atgcttcact
      961 gg
//
