LOCUS       sequence-74210-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination dual luciferase reporter plasmid for checking 
            effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway
            Entry vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pCheck2 Dest (R1-R2)   #BGV129
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
       61 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
      121 gcccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat
      181 cccttcctga ttttctgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
      241 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
      301 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc
      361 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact
      421 gttatctggc ttttagtaag ccggatcctc tagattacgc cccgcctgcc actcatcgca
      481 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      541 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      601 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      661 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
      721 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      781 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      841 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
      901 cggatgagca ttcatcaggc gggcaagaat gtgaataaag g
//
