LOCUS       sequence-74219-e        6339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of FtsZ-mEos2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Buss J, Coltharp C, Huang T, Pohlmeyer C, Wang SC, Hatem C, Xiao J
  TITLE     In vivo organization of the FtsZ-ring by ZapA and ZapB revealed by 
            quantitative super-resolution microscopy.
  JOURNAL   Mol Microbiol. 2013 Sep;89(6):1099-120. doi: 10.1111/mmi.12331. Epub
            2013 Aug 14.
  PUBMED    23859153
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/mmi.12331";
            journalName: "Mol Microbiol"; date: "2013-09"; volume: "89"; issue: 
            "6"; pages: "1099-120"
COMMENT     Sequence Label: pJB042 (FtsZ-mEos2)
FEATURES             Location/Qualifiers
     source          1..6339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(80..94,95..100,101..117,118..124)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    132..148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             167..178
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1378..2058
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     terminator      2107..2201
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2271..2293)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2757..2859
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2860..3519
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2926..2945)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4068..4656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4557..4576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4810..4827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4900..4977
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4978..6060
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4997..5016)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6109..6114,6115..6132,6133..6139)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6147..6163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6171..6187
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6171..6187
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6203..6220)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6203..6219)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6212..6234)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactactagt atatgtttga accaatggaa cttaccaatg acgcggtgat taaagtcatc
      241 ggcgtcggcg gcggcggcgg taatgctgtt gaacacatgg tgcgcgagcg cattgaaggt
      301 gttgaattct tcgcggtaaa taccgatgca caagcgctgc gtaaaacagc ggttggacag
      361 acgattcaaa tcggtagcgg tatcaccaaa ggactgggcg ctggcgctaa tccagaagtt
      421 ggccgcaatg cggctgatga ggatcgcgat gcattgcgtg cggcgctgga aggtgcagac
      481 atggtcttta ttgctgcggg tatgggtggt ggtaccggta caggtgcagc accagtcgtc
      541 gctgaagtgg caaaagattt gggtatcctg accgttgctg tcgtcactaa gcctttcaac
      601 tttgaaggca agaagcgtat ggcattcgcg gagcagggga tcactgaact gtccaagcat
      661 gtggactctc tgatcactat cccgaacgac aaactgctga aagttctggg ccgcggtatc
      721 tccctgctgg atgcgtttgg cgcagcgaac gatgtactga aaggcgctgt gcaaggtatc
      781 gctgaactga ttactcgtcc gggtttgatg aacgtggact ttgcagacgt acgcaccgta
      841 atgtctgaga tgggctacgc aatgatgggt tctggcgtgg cgagcggtga agaccgtgcg
      901 gaagaagctg ctgaaatggc tatctcttct ccgctgctgg aagatatcga cctgtctggc
      961 gcgcgcggcg tgctggttaa catcacggcg ggcttcgatc tgcgtctgga tgagttcgaa
     1021 acggtaggta acaccatccg tgcatttgct tccgacaacg cgactgtggt tatcggtact
     1081 tctcttgacc cggatatgaa tgacgagctg cgcgtaaccg ttgttgcgac aggtatcggc
     1141 atggacaaac gtcctgaaat cactctggtg accaataagc aggttcagca gccagtgatg
     1201 gatcgctacc agcagcatgg gatggctccg ctgacccagg agcagaagcc ggttgctaaa
     1261 gtcgtgaatg acaatgcgcc gcaaactgcg aaagagccgg attatctgga tatcccagca
     1321 ttcctgcgta agcaagctga tggatccgct ggctccgctg ctggttctgg cgaattcatg
     1381 agtgcgatta agccagacat gaagatcaaa ctccgtatgg aaggcaacgt aaacgggcac
     1441 cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat
     1501 cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc
     1561 cattacggca acagggtatt cgccaaatat ccagacaaca tacaagacta ttttaagcag
     1621 tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc
     1681 attgccagaa acgacataac aatggaaggg gacactttct ataataaagt tcgattttat
     1741 ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc
     1801 tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatattca tatggctttg
     1861 ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag
     1921 aagggtgtca agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat
     1981 gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct
     2041 gacaatgcca gacgataagc ggccgctaag ggtcgacctg cagccaagct taattagctg
     2101 agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     2161 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag gggaattcat
     2221 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2281 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2341 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     2401 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     2461 taggttaatg tcatgataat aatggtttct taggatcctg atgtccggcg gtgcttttgc
     2521 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
     2581 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcacccgac
     2641 gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct
     2701 ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat
     2761 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     2821 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     2881 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     2941 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     3001 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     3061 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     3121 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     3181 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     3241 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     3301 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     3361 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     3421 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
     3481 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     3541 tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     3601 attcgaaagc aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta
     3661 tgtctattgc tggtttaccg gtttattgac taccggaagc agtgtgaccg tgtgcttctc
     3721 aaatgcctga ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat
     3781 catttattct gcctcccaga gcctgataaa aacggttagg atcggagtca ggcaactatg
     3841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3901 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3961 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4201 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4681 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4741 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4801 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     4861 cttacgcatc tgtgcggtat ttcacaccgc ataaattccg acaccatcga atggtgcaaa
     4921 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg
     4981 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     5041 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     5101 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     5161 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     5221 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     5281 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     5341 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     5401 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     5461 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     5521 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     5581 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     5641 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     5701 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     5761 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     5821 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     5881 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     5941 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     6001 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6061 gcgcaacgca attaatgtaa gttagctcac tcattaggca ccccaggctt tacactttat
     6121 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6181 ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6241 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6301 gcgaagaggc ccgcaccgat cgcccttccc aacagttgc
//
