LOCUS       sequence-7422-ex        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TLR8-YFP
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4096..4098,4099..4101,4102..4815)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4141..4162)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4402..4421)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4749..4770
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4831..4849)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4832..4849)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4869..4886)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4875..5099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5145..5573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5232..5251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5442..5463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5582..5602)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5587..5917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5768..5903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5830..5849
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5984..6778
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6038..6057)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6648..6667
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6952..7073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6989..7008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7043..7062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7122..7138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7122..7138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7135..7157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7146..7162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7170..7176,7177..7194,7195..7200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7215..7236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7353..7370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7524..8112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7604..7623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8283..9074,9075..9143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8906..8925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9144..9248)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccatgaagga gtcatctttg caaaatagct cctgcagtct gggaaaggag
      961 actaaaaagg aaaacatgtt ccttcagtcg tcaatgctga cctgcatttt cctgctaata
     1021 tctggttcct gtgagttatg cgccgaagaa aatttttcta gaagctatcc ttgtgatgag
     1081 aaaaagcaaa atgactcagt tattgcagag tgcagcaatc gtcgactaca ggaagttccc
     1141 caaacggtgg gcaaatatgt gacagaacta gacctgtctg ataatttcat cacacacata
     1201 acgaatgaat catttcaagg gctgcaaaat ctcactaaaa taaatctaaa ccacaacccc
     1261 aatgtacagc accagaacgg aaatcccggt atacaatcaa atggcttgaa tatcacagac
     1321 ggggcattcc tcaacctaaa aaacctaagg gagttactgc ttgaagacaa ccagttaccc
     1381 caaataccct ctggtttgcc agagtctttg acagaactta gtctaattca aaacaatata
     1441 tacaacataa ctaaagaggg catttcaaga cttataaact tgaaaaatct ctatttggcc
     1501 tggaactgct attttaacaa agtttgcgag aaaactaaca tagaagatgg agtatttgaa
     1561 acgctgacaa atttggagtt gctatcacta tctttcaatt ctctttcaca cgtgccaccc
     1621 aaactgccaa gctccctacg caaacttttt ctgagcaaca cccagatcaa atacattagt
     1681 gaagaagatt tcaagggatt gataaattta acattactag atttaagcgg gaactgtccg
     1741 aggtgcttca atgccccatt tccatgcgtg ccttgtgatg gtggtgcttc aattaatata
     1801 gatcgttttg cttttcaaaa cttgacccaa cttcgatacc taaacctctc tagcacttcc
     1861 ctcaggaaga ttaatgctgc ctggtttaaa aatatgcctc atctgaaggt gctggatctt
     1921 gaattcaact atttagtggg agaaatagcc tctggggcat ttttaacgat gctgccccgc
     1981 ttagaaatac ttgacttgtc ttttaactat ataaagggga gttatccaca gcatattaat
     2041 atttccagaa acttctctaa acttttgtct ctacgggcat tgcatttaag aggttatgtg
     2101 ttccaggaac tcagagaaga tgatttccag cccctgatgc agcttccaaa cttatcgact
     2161 atcaacttgg gtattaattt tattaagcaa atcgatttca aacttttcca aaatttctcc
     2221 aatctggaaa ttatttactt gtcagaaaac agaatatcac cgttggtaaa agatacccgg
     2281 cagagttatg caaatagttc ctcttttcaa cgtcatatcc ggaaacgacg ctcaacagat
     2341 tttgagtttg acccacattc gaacttttat catttcaccc gtcctttaat aaagccacaa
     2401 tgtgctgctt atggaaaagc cttagattta agcctcaaca gtattttctt cattgggcca
     2461 aaccaatttg aaaatcttcc tgacattgcc tgtttaaatc tgtctgcaaa tagcaatgct
     2521 caagtgttaa gtggaactga attttcagcc attcctcatg tcaaatattt ggatttgaca
     2581 aacaatagac tagactttga taatgctagt gctcttactg aattgtccga cttggaagtt
     2641 ctagatctca gctataattc acactatttc agaatagcag gcgtaacaca tcatctagaa
     2701 tttattcaaa atttcacaaa tctaaaagtt ttaaacttga gccacaacaa catttatact
     2761 ttaacagata agtataacct ggaaagcaag tccctggtag aattagtttt cagtggcaat
     2821 cgccttgaca ttttgtggaa tgatgatgac aacaggtata tctccatttt caaaggtctc
     2881 aagaatctga cacgtctgga tttatccctt aataggctga agcacatccc aaatgaagca
     2941 ttccttaatt tgccagcgag tctcactgaa ctacatataa atgataatat gttaaagttt
     3001 tttaactgga cattactcca gcagtttcct cgtctcgagt tgcttgactt acgtggaaac
     3061 aaactactct ttttaactga tagcctatct gactttacat cttcccttcg gacactgctg
     3121 ctgagtcata acaggatttc ccacctaccc tctggctttc tttctgaagt cagtagtctg
     3181 aagcacctcg atttaagttc caatctgcta aaaacaatca acaaatccgc acttgaaact
     3241 aagaccacca ccaaattatc tatgttggaa ctacacggaa acccctttga atgcacctgt
     3301 gacattggag atttccgaag atggatggat gaacatctga atgtcaaaat tcccagactg
     3361 gtagatgtca tttgtgccag tcctggggat caaagaggga agagtattgt gagtctggag
     3421 ctaacaactt gtgtttcaga tgtcactgca gtgatattat ttttcttcac gttctttatc
     3481 accaccatgg ttatgttggc tgccctggct caccatttgt tttactggga tgtttggttt
     3541 atatataatg tgtgtttagc taaggtaaaa ggctacaggt ctctttccac atcccaaact
     3601 ttctatgatg cttacatttc ttatgacacc aaagatgcct ctgttactga ctgggtgata
     3661 aatgagctgc gctaccacct tgaagagagc cgagacaaaa acgttctcct ttgtctagag
     3721 gagagggatt gggacccggg attggccatc atcgacaacc tcatgcagag catcaaccaa
     3781 agcaagaaaa cagtatttgt tttaaccaaa aaatatgcaa aaagctggaa ctttaaaaca
     3841 gctttttact tggctttgca gaggctaatg gatgagaaca tggatgtgat tatatttatc
     3901 ctgctggagc cagtgttaca gcattctcag tatttgaggc tacggcagcg gatctgtaag
     3961 agctccatcc tccagtggcc tgacaacccg aaggcagaag gcttgttttg gcaaactctg
     4021 agaaatgtgg tcttgactga aaatgattca cggtataaca atatgtatgt cgattccatt
     4081 aagcaatacc tcgagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4141 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4201 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4261 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgaagtgctt cgcccgctac
     4321 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4381 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4441 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4501 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4561 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4621 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4681 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     4741 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4801 gagctgtaca agtaatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     4861 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4921 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4981 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5041 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5101 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     5161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5281 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
     5341 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5461 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
     5521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5581 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
     5641 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5761 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5821 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5881 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5941 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6001 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6061 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6121 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6181 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6241 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6301 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6361 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6421 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6481 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6541 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6601 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6661 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6721 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6781 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6841 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6901 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6961 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7021 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7081 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7681 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     7741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7921 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9241 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
