LOCUS       sequence-74259-e         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for ectopic constitutive expression of two mycobacterial proteins in
            one vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pST-2K
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..76)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaggcccagt ctttcgactg agcctttcgt tttagttaac tgatagcttt tacttgtcgt
       61 cgtcgtcctt gtagtcaagc ttaattaaca cgtgctgcag gaattccatt gcgaagtgat
      121 tcctccgggc ccgcatgcgg atcgtgctca tttcgggcgg cgaatctctc ggcgtcgaaa
      181 tgatgtatgc cgtgctggtc gtacacacgg gtactcccag agcctactcg accgagcaca
      241 cgcaggacaa actcgcccac cccgggtggg ttctcccgct cgtcagagac ccttctcaca
      301 ccgggcctgt gcgtgcgaac gcacagatca acgccgggat ggcgtagagc tcactgcgca
      361 gcaccgtggg gatgcggccg atcatcacgt cgcggatcgt gccgcctccc acggccgtga
      421 cggtgccgac gatccaatat tagtactcag ctgggtgaag ggggcg
//
