LOCUS       sequence-74261-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the Gateway selection cassette (ccdB ChlorR) flanked by 
            AttR1 and AttR4 sites for the creation of Destination vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R1-ChlorR-R4   #BGV049
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..154
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(216..521)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(318..337)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggcgaattgg ggatcgaagt taaccccggg caactttgta tagaaaagtt gaacgagaaa
       61 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      121 aatactgtaa aacacaacat atccagtcac tatggactag tggcgcgccg atatcgtcga
      181 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
      241 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
      301 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
      361 accaccgggt aaagttcacg ggggacttta tctgacagca gacgtgcact ggccaggggg
      421 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
      481 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
      541 gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
      601 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
      661 cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
      721 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat
      781 cagtatatat tcttataccg ca
//
