LOCUS       sequence-74265-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-tagged human Erk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  TITLE     cobb lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFLAG-CMV-hErk1
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
       61 cgattacaag gatgacgatg acaagcttgc ggccgcgaat tcaatggcgg cggcggcggc
      121 tcaggggggc gggggcgggg agccccgtag aaccgagggg gtcggcccgg gggtcccggg
      181 ggaggtggag atggtgaagg ggcagccgtt cgacgtgggc ccgcgctaca cgcagttgca
      241 gtacatcggc gagggcgcgt acggcatggt cagctcggcc tatgaccacg tgcgcaagac
      301 tcgcgtggcc atcaagaaga tcagcccctt cgaacatcag acctactgcc agcgcacgct
      361 ccgggagatc cagatcctgc tgcgcttccg ccatgagaat gtcatcggca tccgagacat
      421 tctgcgggcg tccaccctgg aagccatgag agatgtctac attgtgcagg acctgatgga
      481 gactgacctg tacaagttgc tgaaaagcca gcagctgagc aatgaccata tctgctactt
      541 cctctaccag atcctgcggg gcctcaagta catccactcc gccaacgtgc tccaccgaga
      601 tctaaagccc tccaacctgc tcatcaacac cacctgcgac cttaagattt gtgatttcgg
      661 cctggcccgg attgccgatc ctgagcatga ccacaccggc ttcctgacgg agtatgtggc
      721 tacgcgctgg taccgggccc cagagatcat gctgaactcc aagggcta
//
