LOCUS       sequence-74272-e        6492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses the full-length human TET1 gene with a Flag 
            and HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Tahiliani M, Koh KP, Shen Y, Pastor WA, Bandukwala H, Brudno Y, 
            Agarwal S, Iyer LM, Liu DR, Aravind L, Rao A
  TITLE     Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in 
            mammalian DNA by MLL partner TET1.
  JOURNAL   Science. 2009 May 15;324(5929):930-5. Epub 2009 Apr 16.
  PUBMED    19372391
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2009-05-15"; volume: "324(5929)"; pages: "930-5. Epub 2009 
            Apr 16"
COMMENT     Sequence Label: FH-TET1-pEF
FEATURES             Location/Qualifiers
     source          1..6492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggactaca aggacgacga tgacaagctc gatggaggat acccctacga cgtgcccgac
       61 tacgccggag gactcgactc tcgatcccgc catgcaaggc cttccagatt agtcaggaag
      121 gaagatgtaa acaaaaaaaa gaaaaacagc caactacgaa agacaaccaa gggagccaac
      181 aaaaatgtgg catcagtcaa gactttaagc cctggaaaat taaagcaatt aattcaagaa
      241 agagatgtta agaaaaaaac agaacctaaa ccacccgtgc cagtcagaag ccttctgaca
      301 agagctggag cagcacgcat gaatttggat aggactgagg ttctttttca gaacccagag
      361 tccttaacct gcaatgggtt tacaatggcg ctacgaagca cctctcttag caggcgactc
      421 tcccaacccc cactggtcgt agccaaatcc aaaaaggttc cactttctaa gggtttagaa
      481 aagcaacatg attgtgatta taagatactc cctgctttgg gagtaaagca ctcagaaaat
      541 gattcggttc caatgcaaga cacccaagtc cttcctgata tagagactct aattggtgta
      601 caaaatccct ctttacttaa aggtaagagc caagagacaa ctcagttttg gtcccaaaga
      661 gttgaggatt ccaagatcaa tatccctacc cacagtggcc ctgcagctga gatccttcct
      721 gggccactgg aagggacacg ctgtggtgaa ggactattct ctgaagagac attgaatgat
      781 accagtggtt ccccaaaaat gtttgctcag gacacagtgt gtgctccttt tccccaaaga
      841 gcaaccccca aagttacctc tcaaggaaac cccagcattc agttagaaga gttgggttca
      901 cgagtagaat ctcttaagtt atctgattct tacctggatc ccattaaaag tgaacatgat
      961 tgctacccca cctccagtct taataaggtt atacctgact tgaaccttag aaactgcttg
     1021 gctcttggtg ggtctacgtc tcctacctct gtaataaaat tcctcttggc aggctcaaaa
     1081 caagcgaccc ttggtgctaa accagatcat caagaggcct tcgaagctac tgcaaatcaa
     1141 caggaagttt ctgataccac ctctttccta ggacaggcct ttggtgctat cccacatcaa
     1201 tgggaacttc ctggtgctga cccagttcat ggtgaggccc tgggtgagac cccagatcta
     1261 ccagagattc ctggtgctat tccagtccaa ggagaggtct ttggtactat tttagaccaa
     1321 caagaaactc ttggtatgag tgggagtgtt gtcccagact tgcctgtctt ccttcctgtt
     1381 cctccaaatc caattgctac ctttaatgct ccttccaaat ggcctgagcc ccaaagcact
     1441 gtctcatatg gacttgcagt ccagggtgct atacagattt tgcctttggg ctcaggacac
     1501 actcctcaat catcatcaaa ctcagagaaa aattcattac ctccagtaat ggctataagc
     1561 aatgtagaaa atgagaagca ggttcatata agcttcctgc cagctaacac tcaggggttc
     1621 ccattagccc ctgagagagg actcttccat gcttcactgg gtatagccca actctctcag
     1681 gctggtccta gcaaatcaga cagagggagc tcccaggtca gtgtaaccag cacagttcat
     1741 gttgtcaaca ccacagtggt gactatgcca gtgccaatgg tcagtacctc ctcttcttcc
     1801 tataccactt tgctaccgac tttggaaaag aagaaaagaa agcgatgtgg ggtctgtgaa
     1861 ccctgccagc agaagaccaa ctgtggtgaa tgcacttact gcaagaacag aaagaacagc
     1921 catcagatct gtaagaaaag aaaatgtgag gagctgaaaa agaaaccatc tgttgttgtg
     1981 cctctggagg ttataaagga aaacaagagg ccccagaggg aaaagaagcc caaagtttta
     2041 aaggcagatt ttgacaacaa accagtaaat ggccccaagt cagaatccat ggactacagt
     2101 agatgtggtc atggggaaga acaaaaattg gaattgaacc cacatactgt tgaaaatgta
     2161 actaaaaatg aagacagcat gacaggcatc gaggtggaga agtggacaca aaacaagaaa
     2221 tcacagttaa ctgatcacgt gaaaggagat tttagtgcta atgtcccaga agctgaaaaa
     2281 tcgaaaaact ctgaagttga caagaaacga accaaatctc caaaattgtt tgtacaaacc
     2341 gtaagaaatg gcattaaaca tgtacactgt ttaccagctg aaacaaatgt ttcatttaaa
     2401 aaattcaata ttgaagaatt cggcaagaca ttggaaaaca attcttataa attcctaaaa
     2461 gacactgcaa accataaaaa cgctatgagc tctgttgcta ctgatatgag ttgtgatcat
     2521 ctcaagggga gaagtaacgt tttagtattc cagcagcctg gctttaactg cagttccatt
     2581 ccacattctt cacactccat cataaatcat catgctagta tacacaatga aggtgatcaa
     2641 ccaaaaactc ctgagaatat accaagtaaa gaaccaaaag atggatctcc cgttcaacca
     2701 agtctcttat cgttaatgaa agataggaga ttaacattgg agcaagtggt agccatagag
     2761 gccctgactc aactctcaga agccccatca gagaattcct ccccatcaaa gtcagagaag
     2821 gatgaggaat cagagcagag aacagccagt ttgcttaata gctgcaaagc tatcctctac
     2881 actgtaagaa aagacctcca agacccaaac ttacagggag agccaccaaa acttaatcac
     2941 tgtccatctt tggaaaaaca aagttcatgc aacacggtgg ttttcaatgg gcaaactact
     3001 accctttcca actcacatat caactcagct actaaccaag catccacaaa gtcacatgaa
     3061 tattcaaaag tcacaaattc attatctctt tttataccaa aatcaaattc atccaagatt
     3121 gacaccaata aaagtattgc tcaagggata attactcttg acaattgttc caatgatttg
     3181 catcagttgc caccaagaaa taatgaagtg gagtattgca accagttact ggacagcagc
     3241 aaaaaattgg actcagatga tctatcatgt caggatgcaa cccataccca aattgaggaa
     3301 gatgttgcaa cacagttgac acaacttgct tcgataatta agatcaatta tataaaacca
     3361 gaggacaaaa aagttgaaag tacaccaaca agccttgtca catgtaatgt acagcaaaaa
     3421 tacaatcagg agaagggcac aatgcaacag aaaccacctt caagtgtaca caataatcat
     3481 ggttcatcat taacaaaaca aaagaaccca acccagaaaa agacaaaatc caccccatca
     3541 agagatcggc ggaaaaagaa gcccacagtt gtaagttatc aagaaaatga tcggcagaag
     3601 tgggaaaagt tgtcctatat gtatggcaca atatgcgaca tttggatagc atcgaaattt
     3661 caaaattttg ggcaattttg tccacatgat tttcctactg tatttgggaa aatttcttcc
     3721 tcgaccaaaa tatggaaacc actggctcaa acgaggtcca ttatgcaacc caaaacagta
     3781 tttccaccac tcactcagat aaaattacag agatatcctg aatcagcaga ggaaaaggtg
     3841 aaggttgaac cattggattc actcagctta tttcatctta aaacggaatc caacgggaag
     3901 gcattcactg ataaagctta taattctcag gtacagttaa cggtgaatgc caatcagaaa
     3961 gcccatcctt tgacccagcc ctcctctcca cctaaccagt gtgctaacgt gatggcaggc
     4021 gatgaccaaa tacggtttca gcaggttgtt aaggagcaac tcatgcatca gagactgcca
     4081 acattgcctg gtatctctca tgaaacaccc ttaccggagt cagcactaac tctcaggaat
     4141 gtaaatgtag tgtgttcagg tggaattaca gtggtttcta ccaaaagtga agaggaagtc
     4201 tgttcatcca gttttggaac atcagaattt tccacagtgg acagtgcaca gaaaaatttt
     4261 aatgattatg ccatgaactt ctttactaac cctacaaaaa acctagtgtc tataactaaa
     4321 gattctgaac tgcccacctg cagctgtctt gatcgagtta tacaaaaaga caaaggccca
     4381 tattatacac accttggggc aggaccaagt gttgctgctg tcagggaaat catggagaat
     4441 aggtatggtc aaaaaggaaa cgcaataagg atagaaatag tagtgtacac cggtaaagaa
     4501 gggaaaagct ctcatgggtg tccaattgct aagtgggttt taagaagaag cagtgatgaa
     4561 gaaaaagttc tttgtttggt ccggcagcgt acaggccacc actgtccaac tgctgtgatg
     4621 gtggtgctca tcatggtgtg ggatggcatc cctcttccaa tggccgaccg gctatacaca
     4681 gagctcacag agaatctaaa gtcatacaat gggcacccta ccgacagaag atgcaccctc
     4741 aatgaaaatc gtacctgtac atgtcaagga attgatccag agacttgtgg agcttcattc
     4801 tcttttggct gttcatggag tatgtacttt aatggctgta agtttggtag aagcccaagc
     4861 cccagaagat ttagaattga tccaagctct cccttacatg aaaaaaacct tgaagataac
     4921 ttacagagtt tggctacacg attagctcca atttataagc agtatgctcc agtagcttac
     4981 caaaatcagg tggaatatga aaatgttgcc cgagaatgtc ggcttggcag caaggaaggt
     5041 cgtcccttct ctggggtcac tgcttgcctg gacttctgtg ctcatcccca cagggacatt
     5101 cacaacatga ataatggaag cactgtggtt tgtaccttaa ctcgagaaga taaccgctct
     5161 ttgggtgtta ttcctcaaga tgagcagctc catgtgctac ctctttataa gctttcagac
     5221 acagatgagt ttggctccaa ggaaggaatg gaagccaaga tcaaatctgg ggccatcgag
     5281 gtcctggcac cccgccgcaa aaaaagaacg tgtttcactc agcctgttcc ccgttctgga
     5341 aagaagaggg ctgcgatgat gacagaggtt cttgcacata agataagggc agtggaaaag
     5401 aaacctattc cccgaatcaa gcggaagaat aactcaacaa caacaaacaa cagtaagcct
     5461 tcgtcactgc caaccttagg gagtaacact gagaccgtgc aacctgaagt aaaaagtgaa
     5521 accgaacccc attttatctt aaaaagttca gacaacacta aaacttattc gctgatgcca
     5581 tccgctcctc acccagtgaa agaggcatct ccaggcttct cctggtcccc gaagactgct
     5641 tcagccacac cagctccact gaagaatgac gcaacagcct catgcgggtt ttcagaaaga
     5701 agcagcactc cccactgtac gatgccttcg ggaagactca gtggtgccaa tgcagctgct
     5761 gctgatggcc ctggcatttc acagcttggc gaagtggctc ctctccccac cctgtctgct
     5821 cctgtgatgg agcccctcat taattctgag ccttccactg gtgtgactga gccgctaacg
     5881 cctcatcagc caaaccacca gccctccttc ctcacctctc ctcaagacct tgcctcttct
     5941 ccaatggaag aagatgagca gcattctgaa gcagatgagc ctccatcaga cgaaccccta
     6001 tctgatgacc ccctgtcacc tgctgaggag aaattgcccc acattgatga gtattggtca
     6061 gacagtgagc acatcttttt ggatgcaaat attggtgggg tggccatcgc acctgctcac
     6121 ggctcggttt tgattgagtg tgcccggcga gagctgcacg ctaccactcc tgttgagcac
     6181 cccaaccgta atcatccaac ccgcctctcc cttgtctttt accagcacaa aaacctaaat
     6241 aagccccaac atggttttga actaaacaag attaagtttg aggctaaaga agctaagaat
     6301 aagaaaatga aggcctcaga gcaaaaagac caggcagcta atgaaggtcc agaacagtcc
     6361 tctgaagtaa atgaattgaa ccaaattcct tctcataaag cattaacatt aacccatgac
     6421 aatgttgtca ccgtgtcccc ttatgctctc acacacgttg cggggcccta taaccattgg
     6481 gtctgatcta ga
//
