LOCUS       sequence-74274-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH82094
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..69
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(70..87)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(70..86)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(79..101)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(295..314)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    451..475
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
       61 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
      241 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      301 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
      361 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgacgg tctcagggac
      421 acgaagtgat ccgtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
      481 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
      541 tcgtccattt gtatgtgcca gccgcctaga tgtggcgcaa cgatgccggc gacaagcagg
      601 agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt
      661 atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga
      721 aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca
      781 ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg
      841 caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac
      901 tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc
      961 gtgcgccccg cgaaaccttc cagtccgtcg gctcg
//
