LOCUS       sequence-74283-e       10068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses VSVG fused to UVR8 for light-triggered trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Chen D, Gibson ES, Kennedy MJ
  TITLE     A light-triggered protein secretion system.
  JOURNAL   J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119.
  PUBMED    23671313
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-05-13- 13"; volume: "201"; 
            issue: "4"; pages: "631-40"
COMMENT     Sequence Label: VSVG-YFP-2xUVR8
FEATURES             Location/Qualifiers
     source          1..10068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     477..497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     523..547
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             join(678..725,726..2210)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein, 
                     thermosensitive tsO45 mutant"
                     /label=VSV-G tsO45
                     /note="thermosensitive variant of VSV-G Orsay"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAAIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNDICPTVHNSTTWHSDYKVKGLCDSNLISTDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGDKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPVFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSLGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDETLFFGDTGLSKNPIEFVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIYLCIKLKHTKKRQIYTDIEMNRLGK"
     CDS             2223..2252
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2265..2274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2271..2273,2274..2276,2277..2984)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(2316..2337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2577..2596)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2924..2945
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3018..3027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5712..5738
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          5770..6342
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(6474..6493)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6539..6594
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6593..6612)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      6947..7082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7188..7205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7359..7947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7439..7458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8741..8760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8991..9095)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9163..9181
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9219..9241)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9341..9360
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9427..9882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9564..9585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9776..9795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10012..10034
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10026..10043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10027..10043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10050..10068
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 caagcttcga attcgccatg aagtgccttt tgtacttagc ttttttattc atcggggtga
      721 attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt
      781 ccaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca
      841 cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt
      901 gtcatgcttc caaatgggtc actacttgtg atttccgctg gtacggaccg aagtatataa
      961 cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa
     1021 cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg
     1081 tgacggatgc tgaagcagcg attgtccagg tgactcctca ccatgtgctt gttgatgaat
     1141 acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat gacatatgcc
     1201 ccactgtcca taactccaca acctggcatt ccgactataa ggtcaaaggg ctatgtgatt
     1261 ctaacctcat ttccacggac atcaccttct tctcagagga cggagagcta tcatccctag
     1321 gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga gacaaggcct
     1381 gcaaaatgca gtactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga
     1441 tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta
     1501 tctctgctcc atctcagacc tcagtggatg taagtctcat tcaggacgtt gagaggatct
     1561 tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt cccatctctc
     1621 cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgtc tttaccataa
     1681 tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa
     1741 tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg
     1801 actgggctcc atatgaagac gtggaaattg gacccaatgg agttctgagg accagtttag
     1861 gatataagtt tcctttatat atgattggac atggtatgtt ggactccgat cttcatctta
     1921 gctcaaaggc tcaggtgttt gaacatcctc acattcaaga cgctgcttcg cagcttcctg
     1981 atgatgagac tttatttttt ggtgatactg ggctatccaa aaatccaatc gagtttgtag
     2041 aaggttggtt cagtagttgg aagagctcta ttgcctcttt tttctttatc atagggttaa
     2101 tcattggact attcttggtt ctccgagttg gtatttatct ttgcattaaa ttaaagcaca
     2161 ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag gatctccccg
     2221 ccgaacagaa actcatctcc gaagaagacc tggatccacc ggtcgccacc atggtgagca
     2281 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2341 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2401 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2461 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2521 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2581 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2641 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2701 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2761 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2821 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     2881 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2941 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacagtggt ggtggagggg
     3001 ggggactcag atctcgagcc accatggcgg aggatatggc tgccgacgaa gttacggctc
     3061 ctcctcgtaa ggttcttatc atctccgctg gtgctagcca ctccgtcgct cttctctctg
     3121 gtgacattgt ttgttcttgg ggtcgaggag aggatggaca gttaggtcat ggcgatgcag
     3181 aggatcgacc ttctccgact cagcttagcg ctttagatgg ccaccaaatt gtttccgtta
     3241 cctgtggtgc tgatcacact gttgcttatt cacaatcagg catggaagtc tacagttggg
     3301 gatggggtga ttttgggaga ttaggccatg gtaactcaag cgacttgttt actccgctac
     3361 caatcaaagc attgcacggt attcggatca agcagattgc ttgtggggat agtcattgtt
     3421 tggctgtcac tatggaagga gaggtccaga gttggggccg caaccagaat ggtcaacttg
     3481 gtctggggga caccgaagat tctctagtgc ctcagaagat tcaagccttt gagggaatac
     3541 gaatcaaaat ggttgctgct ggtgcagaac acactgctgc agttacagaa gatggtgacc
     3601 tctatggatg gggctgggga agatacggaa atttgggatt aggtgaccgg actgaccgct
     3661 tagttcctga aagagttacc tctactggtg gtgagaaaat gtcaatggtt gcttgtggat
     3721 ggcggcacac aatatcagtt tcctactctg gagcattgta tacttatgga tggagcaaat
     3781 atggacagct aggacatgga gacttggagg atcaccttat tcctcacaaa ctggaagcac
     3841 tgagcaacag ttttatctcc cagatttcgg gaggttggag acatacaatg gcattgactt
     3901 cagatggaaa actatatgga tggggttgga ataagtttgg acaagtagga gtcggcaata
     3961 atttagatca gtgttctcct gtgcaagtgc gatttcccga tgatcagaaa gtagttcaag
     4021 tctcatgtgg atggagacat accttggctg tcactgaaag aaataacgtg tttgcttggg
     4081 gtagaggtac aaatggacag ctcggcattg gagagtcggt tgacaggaac tttcccaaga
     4141 ttatagaggc actcagcgtc gatggagcaa gtggacaaca tatagaatct tctaatatcg
     4201 atccatcttc agggaaaagc tgggtgtcgc ctgcagagag atatgcagtt gttcctgatg
     4261 aaacgggcct aacggatggt tcaagcaaag gtaatggagg tgatatcagt gttccacaaa
     4321 ctgatgtcaa gcgtgtacga attaagcttg ggggcggagg cgcggaggat atggctgccg
     4381 acgaagttac ggctcctcct cgtaaggttc ttatcatctc cgctggtgct agccactccg
     4441 tcgctcttct ctctggtgac attgtttgtt cttggggtcg aggagaggat ggacagttag
     4501 gtcatggcga tgcagaggat cgaccttctc cgactcagct tagcgcttta gatggccacc
     4561 aaattgtttc cgttacctgt ggtgctgatc acactgttgc ttattcacaa tcaggcatgg
     4621 aagtctacag ttggggatgg ggtgattttg ggagattagg ccatggtaac tcaagcgact
     4681 tgtttactcc gctaccaatc aaagcattgc acggtattcg gatcaagcag attgcttgtg
     4741 gggatagtca ttgtttggct gtcactatgg aaggagaggt ccagagttgg ggccgcaacc
     4801 agaatggtca acttggtctg ggggacaccg aagattctct agtgcctcag aagattcaag
     4861 cctttgaggg aatacgaatc aaaatggttg ctgctggtgc agaacacact gctgcagtta
     4921 cagaagatgg tgacctctat ggatggggct ggggaagata cggaaatttg ggattaggtg
     4981 accggactga ccgcttagtt cctgaaagag ttacctctac tggtggtgag aaaatgtcaa
     5041 tggttgcttg tggatggcgg cacacaatat cagtttccta ctctggagca ttgtatactt
     5101 atggatggag caaatatgga cagctaggac atggagactt ggaggatcac cttattcctc
     5161 acaaactgga agcactgagc aacagtttta tctcccagat ttcgggaggt tggagacata
     5221 caatggcatt gacttcagat ggaaaactat atggatgggg ttggaataag tttggacaag
     5281 taggagtcgg caataattta gatcagtgtt ctcctgtgca agtgcgattt cccgatgatc
     5341 agaaagtagt tcaagtctca tgtggatgga gacatacctt ggctgtcact gaaagaaata
     5401 acgtgtttgc ttggggtaga ggtacaaatg gacagctcgg cattggagag tcggttgaca
     5461 ggaactttcc caagattata gaggcactca gcgtcgatgg agcaagtgga caacatatag
     5521 aatcttctaa tatcgatcca tcttcaggga aaagctgggt gtcgcctgca gagagatatg
     5581 cagttgttcc tgatgaaacg ggcctaacgg atggttcaag caaaggtaat ggaggtgata
     5641 tcagtgttcc acaaactgat gtcaagcgtg tacgaattgg gtcgacggta ccgcgggccc
     5701 gggatactag ttatccgtac gacgtaccag actacgcata agaaaagtga ggatcctgag
     5761 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
     5821 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
     5881 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
     5941 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
     6001 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
     6061 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
     6121 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
     6181 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     6241 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     6301 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     6361 gttgttgtgc tgtctcatca ttttggcaaa ggattcactc ctcaggtgca ggctgcctat
     6421 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     6481 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     6541 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     6601 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     6661 gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta
     6721 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     6781 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     6841 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     6901 tcttctctta tgaagatccc tcgaggagct ttttgcaaaa gccctaggcc tccaaaaaag
     6961 cctcttcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     7021 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     7081 atgggcggag ttaggggcgg gactatggtt gctgactaat tgctgcatta atgaatcggc
     7141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8761 agaactttaa aagtgctcat cattgccacc tccacctgaa aacgttcttc ggggcgaaaa
     8821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9001 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9121 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9181 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9241 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9301 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9361 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9421 atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     9481 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     9541 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     9601 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     9661 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     9721 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     9781 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     9841 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca
     9901 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9961 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    10021 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagg
//
