LOCUS       sequence-74288-e       10023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses VSVG fused to UVR8 for light-triggered trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Chen D, Gibson ES, Kennedy MJ
  TITLE     A light-triggered protein secretion system.
  JOURNAL   J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119.
  PUBMED    23671313
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-05-13- 13"; volume: "201"; 
            issue: "4"; pages: "631-40"
COMMENT     Sequence Label: VSVG-mEOS2-2xUVR8
FEATURES             Location/Qualifiers
     source          1..10023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     477..497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     523..547
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             join(678..725,726..2210)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein, 
                     thermosensitive tsO45 mutant"
                     /label=VSV-G tsO45
                     /note="thermosensitive variant of VSV-G Orsay"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAAIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNDICPTVHNSTTWHSDYKVKGLCDSNLISTDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGDKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPVFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSLGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDETLFFGDTGLSKNPIEFVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIYLCIKLKHTKKRQIYTDIEMNRLGK"
     CDS             2223..2252
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2259..2933
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="SAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     regulatory      2973..2982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5667..5693
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          5725..6297
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(6429..6448)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6494..6549
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6548..6567)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      6902..7037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7143..7160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7314..7902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7394..7413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8696..8715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8946..9050)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9118..9136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9174..9196)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9296..9315
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9382..9837)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9519..9540)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9731..9750
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9967..9989
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9981..9998
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9982..9998
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10005..10023
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 caagcttcga attcgccatg aagtgccttt tgtacttagc ttttttattc atcggggtga
      721 attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt
      781 ccaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca
      841 cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt
      901 gtcatgcttc caaatgggtc actacttgtg atttccgctg gtacggaccg aagtatataa
      961 cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa
     1021 cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg
     1081 tgacggatgc tgaagcagcg attgtccagg tgactcctca ccatgtgctt gttgatgaat
     1141 acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat gacatatgcc
     1201 ccactgtcca taactccaca acctggcatt ccgactataa ggtcaaaggg ctatgtgatt
     1261 ctaacctcat ttccacggac atcaccttct tctcagagga cggagagcta tcatccctag
     1321 gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga gacaaggcct
     1381 gcaaaatgca gtactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga
     1441 tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta
     1501 tctctgctcc atctcagacc tcagtggatg taagtctcat tcaggacgtt gagaggatct
     1561 tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt cccatctctc
     1621 cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgtc tttaccataa
     1681 tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa
     1741 tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg
     1801 actgggctcc atatgaagac gtggaaattg gacccaatgg agttctgagg accagtttag
     1861 gatataagtt tcctttatat atgattggac atggtatgtt ggactccgat cttcatctta
     1921 gctcaaaggc tcaggtgttt gaacatcctc acattcaaga cgctgcttcg cagcttcctg
     1981 atgatgagac tttatttttt ggtgatactg ggctatccaa aaatccaatc gagtttgtag
     2041 aaggttggtt cagtagttgg aagagctcta ttgcctcttt tttctttatc atagggttaa
     2101 tcattggact attcttggtt ctccgagttg gtatttatct ttgcattaaa ttaaagcaca
     2161 ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag gatctccccg
     2221 ccgaacagaa actcatctcc gaagaagacc tggatccaag tgcgattaag ccagacatga
     2281 agatcaaact ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
     2341 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
     2401 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
     2461 ccaaatatcc agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
     2521 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
     2581 tggaagggga cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg
     2641 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
     2701 gtgatggagt gctgacgggt gatattcata tggctttgtt gcttgaagga aatgcccatt
     2761 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
     2821 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
     2881 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgactgtaca
     2941 gtggtggtgg agggggggga ctcagatctc gagccaccat ggcggaggat atggctgccg
     3001 acgaagttac ggctcctcct cgtaaggttc ttatcatctc cgctggtgct agccactccg
     3061 tcgctcttct ctctggtgac attgtttgtt cttggggtcg aggagaggat ggacagttag
     3121 gtcatggcga tgcagaggat cgaccttctc cgactcagct tagcgcttta gatggccacc
     3181 aaattgtttc cgttacctgt ggtgctgatc acactgttgc ttattcacaa tcaggcatgg
     3241 aagtctacag ttggggatgg ggtgattttg ggagattagg ccatggtaac tcaagcgact
     3301 tgtttactcc gctaccaatc aaagcattgc acggtattcg gatcaagcag attgcttgtg
     3361 gggatagtca ttgtttggct gtcactatgg aaggagaggt ccagagttgg ggccgcaacc
     3421 agaatggtca acttggtctg ggggacaccg aagattctct agtgcctcag aagattcaag
     3481 cctttgaggg aatacgaatc aaaatggttg ctgctggtgc agaacacact gctgcagtta
     3541 cagaagatgg tgacctctat ggatggggct ggggaagata cggaaatttg ggattaggtg
     3601 accggactga ccgcttagtt cctgaaagag ttacctctac tggtggtgag aaaatgtcaa
     3661 tggttgcttg tggatggcgg cacacaatat cagtttccta ctctggagca ttgtatactt
     3721 atggatggag caaatatgga cagctaggac atggagactt ggaggatcac cttattcctc
     3781 acaaactgga agcactgagc aacagtttta tctcccagat ttcgggaggt tggagacata
     3841 caatggcatt gacttcagat ggaaaactat atggatgggg ttggaataag tttggacaag
     3901 taggagtcgg caataattta gatcagtgtt ctcctgtgca agtgcgattt cccgatgatc
     3961 agaaagtagt tcaagtctca tgtggatgga gacatacctt ggctgtcact gaaagaaata
     4021 acgtgtttgc ttggggtaga ggtacaaatg gacagctcgg cattggagag tcggttgaca
     4081 ggaactttcc caagattata gaggcactca gcgtcgatgg agcaagtgga caacatatag
     4141 aatcttctaa tatcgatcca tcttcaggga aaagctgggt gtcgcctgca gagagatatg
     4201 cagttgttcc tgatgaaacg ggcctaacgg atggttcaag caaaggtaat ggaggtgata
     4261 tcagtgttcc acaaactgat gtcaagcgtg tacgaattaa gcttgggggc ggaggcgcgg
     4321 aggatatggc tgccgacgaa gttacggctc ctcctcgtaa ggttcttatc atctccgctg
     4381 gtgctagcca ctccgtcgct cttctctctg gtgacattgt ttgttcttgg ggtcgaggag
     4441 aggatggaca gttaggtcat ggcgatgcag aggatcgacc ttctccgact cagcttagcg
     4501 ctttagatgg ccaccaaatt gtttccgtta cctgtggtgc tgatcacact gttgcttatt
     4561 cacaatcagg catggaagtc tacagttggg gatggggtga ttttgggaga ttaggccatg
     4621 gtaactcaag cgacttgttt actccgctac caatcaaagc attgcacggt attcggatca
     4681 agcagattgc ttgtggggat agtcattgtt tggctgtcac tatggaagga gaggtccaga
     4741 gttggggccg caaccagaat ggtcaacttg gtctggggga caccgaagat tctctagtgc
     4801 ctcagaagat tcaagccttt gagggaatac gaatcaaaat ggttgctgct ggtgcagaac
     4861 acactgctgc agttacagaa gatggtgacc tctatggatg gggctgggga agatacggaa
     4921 atttgggatt aggtgaccgg actgaccgct tagttcctga aagagttacc tctactggtg
     4981 gtgagaaaat gtcaatggtt gcttgtggat ggcggcacac aatatcagtt tcctactctg
     5041 gagcattgta tacttatgga tggagcaaat atggacagct aggacatgga gacttggagg
     5101 atcaccttat tcctcacaaa ctggaagcac tgagcaacag ttttatctcc cagatttcgg
     5161 gaggttggag acatacaatg gcattgactt cagatggaaa actatatgga tggggttgga
     5221 ataagtttgg acaagtagga gtcggcaata atttagatca gtgttctcct gtgcaagtgc
     5281 gatttcccga tgatcagaaa gtagttcaag tctcatgtgg atggagacat accttggctg
     5341 tcactgaaag aaataacgtg tttgcttggg gtagaggtac aaatggacag ctcggcattg
     5401 gagagtcggt tgacaggaac tttcccaaga ttatagaggc actcagcgtc gatggagcaa
     5461 gtggacaaca tatagaatct tctaatatcg atccatcttc agggaaaagc tgggtgtcgc
     5521 ctgcagagag atatgcagtt gttcctgatg aaacgggcct aacggatggt tcaagcaaag
     5581 gtaatggagg tgatatcagt gttccacaaa ctgatgtcaa gcgtgtacga attgggtcga
     5641 cggtaccgcg ggcccgggat actagttatc cgtacgacgt accagactac gcataagaaa
     5701 agtgaggatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
     5761 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
     5821 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
     5881 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
     5941 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
     6001 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
     6061 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
     6121 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc
     6181 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
     6241 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     6301 ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaaggatt cactcctcag
     6361 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     6421 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     6481 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     6541 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     6601 ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata
     6661 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     6721 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     6781 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     6841 agtcatagct gtccctcttc tcttatgaag atccctcgag gagctttttg caaaagccct
     6901 aggcctccaa aaaagcctct tcactacttc tggaatagct cagaggccga ggcggcctcg
     6961 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     7021 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgctg
     7081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7741 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8701 accgcgccac atagcagaac tttaaaagtg ctcatcattg ccacctccac ctgaaaacgt
     8761 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8821 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8881 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8941 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9001 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9061 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     9121 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     9181 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     9241 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     9301 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9361 aggagaaaat accgcatcag gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa
     9421 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9481 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9541 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9601 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9661 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9721 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9781 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc
     9841 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9901 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9961 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
    10021 agg
//
