LOCUS       sequence-74302-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and 
            D2. URA3 was derived from S288c and extends from 243 bp upstream of 
            the start codon to 79 bp downstream of the stop codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Horecka J, Davis RW
  TITLE     The 50:50 method for PCR-based seamless genome editing in yeast.
  JOURNAL   Yeast. 2014 Mar;31(3):103-12. doi: 10.1002/yea.2992. Epub 2013 Dec 
            13.
  PUBMED    24639370
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2992"; 
            journalName: "Yeast"; date: "2014-03"; volume: "31"; issue: "3"; 
            pages: "103-12"
COMMENT     Sequence Label: pJH136
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        107..323
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag
       61 ccccgtacgc tgcaggtcga ctgtggtttc agggtccata aagcttttca attcatcttt
      121 tttttttttg ttcttttttt tgattccggt ttctttgaaa tttttttgat tcggtaatct
      181 ccgagcagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
      241 gtggtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
      301 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
      361 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
      421 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
      481 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
      541 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
      601 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
      661 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
      721 tgaagcaggc ggcggaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
      781 tgtcatgcaa gggctcccta gctactggag aatatactaa gggtactgtt gacattgcga
      841 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
      901 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
      961 tgggtcaaca gtataaa
//
