LOCUS       sequence-74304-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and 
            D2. URA3 was derived from S288c and extends from 243 bp upstream of 
            the start codon to 79 bp downstream of the stop codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Horecka J, Davis RW
  TITLE     The 50:50 method for PCR-based seamless genome editing in yeast.
  JOURNAL   Yeast. 2014 Mar;31(3):103-12. doi: 10.1002/yea.2992. Epub 2013 Dec 
            13.
  PUBMED    24639370
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2992"; 
            journalName: "Yeast"; date: "2014-03"; volume: "31"; issue: "3"; 
            pages: "103-12"
COMMENT     Sequence Label: pJH136
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..52
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     37..53
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
       61 tgatatcgaa ttcctgcagc ccgggggatc cgcccatcga tgaattcgag ctcgggtaat
      121 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
      181 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
      241 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
      301 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
      361 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
      421 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
      481 gcaatgtcaa cagtaccctt agtatattct ccagtagcta gggagccctt gcatgacaat
      541 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttccgccgc ctgcttcaaa
      601 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
      661 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
      721 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
      781 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
      841 cctaatgctt caactaactc cagtaattcc ttggtg
//
