LOCUS       sequence-7431-ex       10259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Wulfkuhle JD, Donina IE, Stark NH, Pope RK, Pestonjamasp KN, 
            Niswonger ML, Luna EJ
  TITLE     Domain analysis of supervillin, an F-actin bundling plasma membrane 
            protein with functional nuclear localization signals.
  JOURNAL   J Cell Sci. 1999 Jul:112 (Pt 13):2125-36. doi: 
            10.1242/jcs.112.13.2125
  PUBMED    10362542
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            1999 Jul:112 (Pt 13):2125-36. doi: 10.1242/jcs.112.13.2125"
COMMENT     Sequence Label: EGFP-supervillin
FEATURES             Location/Qualifiers
     source          1..10259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7047..7168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7084..7103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7138..7157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7175..7630)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7312..7333)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7524..7543
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7657..7761
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7763..8120
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7763..7954
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7786..7806)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7971..8106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8155..8949
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8155..8949
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8209..8228)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8819..8838
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9137..9156)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9181..9228
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9557..10145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10046..10065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 ttcgatgctc agcttttagc tgcccttcct aaagttgcag aattaagaaa aatctttgaa
     1441 ccaaagagga aagaattttt agaaatgaaa agaaaagaaa gaattgcccg gcgcttagaa
     1501 ggaattgaaa ccgacacgca gcccatcctc ttgcagagct gcacgggctt ggtgactcat
     1561 cgcctgctgg aggaggacac acccagatac atgcgtgcca cagacccggc cagcccgcac
     1621 attggtcgat caaatgaaga agaagaaact tcagattctt cactagaaaa gcagactcga
     1681 tccaaacagt gcacagaaac ctcaggcatc catgctgact cgccctacag ttcaggcatc
     1741 atggacaccc agagcctcga gtccaaagcc gaaagaatcg ccaggtacaa agcagagaga
     1801 agacggcagc tggcagaaaa gtatgggctc accctggatc cggaagcaga ctctgaaact
     1861 ccatctcgat acagcaggtc cagaaaggac cccgaggctg cggagaaaag gggagtaaga
     1921 agcgagcgat cggccgagtc cagcagagac gccggctcat cctactccag gactgagctc
     1981 tcggggctca ggacctgcgt ggctgagtcc aaggactang gcctgcaccg gagtgacggt
     2041 gtttccgaca cagaggtgct gctcaatgca gaaaaccaaa gacgcggtca agagcccagt
     2101 gccaccgggc tggcccgaga cctgcccctt gcaggggagg tctcctcttc tttctcattc
     2161 tccgggcgag actctgccct cggtgaagtg ccaaggtccc caaaggcagt gcacagcctg
     2221 cccagtccat cgcctgggca gccggcctcc ccaagccact ccaccagtga cctgccactc
     2281 cctgcggagg ctcgagccag gtcaacttca aattcagaaa tgccagctgc cgaggatgaa
     2341 gaaaaggtcg atgagcgagc gaggctgagc gtcgctgcta agaggctgct cttcagggag
     2401 atggagaagt cgtttgacga gaagagcgtc ccaaagcgcc gctcccgcaa tgcggccgtg
     2461 gagcagaggc tgcggcgcct ccaggaccgg tcccacacgc agcccgtcac caccgaggag
     2521 gtggtcattg cagccacatt gcaggcgtct gctcaccaaa aggcactagc cagagaccag
     2581 acaaacgaga gcaaagattc tgctgagcag ggagaacctg actcctccac tctgagctta
     2641 gcggagaagc tggccttgtt taacaaacta tctcagccgg tctcaaaagc catttcaaca
     2701 cggaacagat tagacatgag acagaggagg atgaatgctc gttatcaaac ccagccggtc
     2761 acgcttggag aagtggaaca ggtgcagagt ggcaagctca tggctttctc tcccaccatt
     2821 aacacttccg tgtccaccgt ggcatctacc gtccccccca tgtatgcagg aaatcttcgg
     2881 acaaagccac ttccagatga cagctttggt gccactgagc agaagtttgc ttcttcgcta
     2941 gaaaactccg actccccagt tagaagcatc ctgaaatccc aaggctggca gccctcggtc
     3001 gagggtgctg ggagcaaagc aatgttgaga gaatttgaag agacagaacg caagggaggc
     3061 ttgacaggtg gagatggcgg ggttacaaag tatgggtcct tcgaggaagc agagctgtcc
     3121 taccccgtcc tcagcagagt ccgggaggga gacaaccata aagaggccat ctatgccctt
     3181 ccgaggaaag gaagcctgga gctcgcccat cctcccatcg cccagcttgg tgatgacctg
     3241 aaggaatttt ccaccccaaa gagcaccatg caggcaagcc cagactggaa ggagaggcag
     3301 ctctttgaag agaaggtgga cttggagaat gtcacaaaga ggaagttttc gctgaaagcc
     3361 gcggagttcg gggaacccac ttctgagcag accggcgcgg ctgctgggaa accggctgct
     3421 ccgactgcaa ccccggtgtc ctggaagccg caggatccct ccgaacagcc tcaggagaag
     3481 cggtatcaga gcccgtgtgc gatgtttgct gctggagaga tcaaagcccc ggcggtggag
     3541 ggcagcctcg actcccccag caaaaccatg tccattaaag aaagattagc actgttgaag
     3601 aagagcggtg aggaagactg gagaaacaga ctcaacagaa agcaggaata cggcaaagcc
     3661 tccatcacca gcagcctgca catccaagag acggagcagt cactcaagaa gaagcgggtc
     3721 acagaaagtc gagagagcca gatgactatt gaagagagaa agcatctcat caccgtgaga
     3781 gaggacgcct ggaagaccag gggcaaagga gcagccaacg actctaccca gtttactgtg
     3841 gccggcagga tggtgaagcg aggtctggcg tcccccactg ccatcacccc ggtagcatcc
     3901 cctgtttcca gcaaagcaag gggcactaca ccagtttcca gacccctgga agatatcgaa
     3961 gccagaccag acatgcagtt agagtcggac ctcaagctgg acaggctgga aaccttccta
     4021 agaaggctga ataacaaagt tggtgggatg caagaaacag tcctcactgt cactgggaaa
     4081 tcggtgaaag aggtgatgaa gccggacgat gatgaaacct ttgccaagtt ttaccgcagc
     4141 gtggactcct ctctaccgag aagccccgta gagctggacg aggatttcga tgtcattttt
     4201 gatccttatg cccccaggtt gacatcctcg gtggctgagc acaagcgtgc cgtgcggccc
     4261 aagcgccggg tccaggcttc caaaaacccc ctgaagatgc tggccgcgag ggaagatctc
     4321 ctgcaggagt acactgagca gagactgaac gtggccttcg tggagtcaaa gcggatgaaa
     4381 gtcgaaaagt tgtccgccaa ctccagcttc tcagaagtca ccctggcggg gttagccagc
     4441 aaagaaaact tcagcaacgt cagcctgcgg agcgtcaacc tgacggaaca gaattccaac
     4501 aacagtgcag tgccctacaa gaagctgatg ctgctgcagg ttaaaggaag aagacacgtg
     4561 cagacgcggc tggtggagcc tcgcgccccc tccctcaaca gcggggactg cttcctcctg
     4621 ctctcgcccc atcactgctt cctgtgggtc ggagagttcg ccaacgtgat cgagaaggcg
     4681 aaggcctcag aacttgcgag tttaattcag accaagaggg aacttggttg tagagcaact
     4741 tacatccaga ctgttgaaga aggaattaat acacacacac atgcagccaa agacttctgg
     4801 aagcttctgg gcggccaagc cagttaccaa tccgccggag acccaaagga ggatgagctc
     4861 tatgaaacgg ccataataga gaccaactgc atttaccgtc tgatggatga caaactcgtt
     4921 cctgatgatg actactgggg gaagatccca aagtgctccc tcctgcaatc aaaagaggta
     4981 ctggtgtttg attttgggag tgaagtttac gtgtggcatg gaaaagaagt cacattagca
     5041 caacggaaaa tcgcctttca gctggcaaag cacttgtgga atggaacctt tgactacgag
     5101 aattgtgaca ttaacccgct ggatcctggg gaatgcaatc ccctcattcc caggaaagga
     5161 caggggcggc ccgactgggc aatatttggg agacttacag aacacaatga gacgatcttg
     5221 ttcaaagaga aattccttga ttggacggaa ctgaagagac ctaacgagaa gaacgccagc
     5281 gaactcgcgc agcacaagga cgatgcccgg gcagaggtca agccttacga cgtgacgcgg
     5341 atggtgcccg tgccccagac aacagccggc accgtgctgg atggggtcaa cgtgggccga
     5401 ggctacgggc tggtggaagg ggacgaccgg aggcagttcg agatcgccag catctcagtg
     5461 gacgtctggc acatcctgga gttcgactac agcaggctcc ccaagcagag catcgggcag
     5521 ttccacgaag gcgatgccta cgtggtgaag tggaagttca tcgtgagcac tgcagtggga
     5581 agccggcaga agggggagca ctcggtcagg gtggctggca aagagaaatg tgtctacttc
     5641 ttctggcaag gccgccagtc gaccgtgagt gagaagggca catcagctct gatgacagtg
     5701 gagctggacg aggaaagggg ggcccaggtc caggtcctgc agggcaagga gcccccctgt
     5761 ttcctgcagt gcttccaggg ggggatggtg gtccactccg ggagacggga ggaggaggaa
     5821 gagaacacac agagcgagtg gcggctgtac tgtgtgcgcg gagaggtgcc tgtggagggg
     5881 aacttgctgg aggtggcctg tcactgcagc agcctcaggt ctaggacgtc catggttgtc
     5941 ctcaacgtcc acaaagccct catctacctg tggcatggat gcaaagccca ggcccacacg
     6001 aaggaggtcg gacggactgc agccaataaa atcaaggacc aatgtcccct ggaggccggg
     6061 ctgcacagta gcagcaaagt gacaattcat gagtgtgatg aaggctcgga gcccctgggg
     6121 ttctgggacg ctctggggag gagagaccgg aaagcctacg actgcatgct tcaagatcct
     6181 ggaaatttta acttcacacc ccgcctgttc atcctcagca gctcctctgg tgacttctca
     6241 gccacagagt tcatgtatcc cgcccgagac ccctctgtgg tcaactctat gcccttcctg
     6301 caggaagacc tatacagtgc cccacagcca gcactcttcc ttgttgacaa tcaccacgag
     6361 gtgtacctct ggcaaggctg gtggcccatc gagaacaaga tcacgggctc cgcgcgcatc
     6421 cgctgggcct cggaccggaa gagcgccatg gagacagtgc tgcagtactg ccgagggaaa
     6481 aacctgaaga agccgccccc caagtcttac ctcatccacg ctggcctgga acccctgacc
     6541 ttcaccaaca tgttccccag ctgggagcac agagaagaca ttgcagagat cacggagatg
     6601 gacacggaag tttcaaatca gatcacccta gtggaggatg tcttagccaa actgtgtaag
     6661 accatttacc cactggccga cctcctagcc aggccgctcc ctgagggagt tgaccctctg
     6721 aagctggaga tctatctcac cgacgaggac ttcgagtttg cactagacat gacccgagac
     6781 gagtacaatg cactgcccgc ttggaagcag gtgaacctga agaaagcgaa aggtctgttc
     6841 tgagtcagga gatgctggag gaacctgggt ggtcactttc aatactgatg gaccaggaaa
     6901 atggatgttt aatcgaattc ccgcggcctt ctagataact gatcataatc agccatacca
     6961 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     7021 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     7081 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7141 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     7201 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7261 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7321 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7381 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     7441 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7501 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     7561 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     7621 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7681 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7741 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     7801 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7861 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7921 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7981 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8041 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8101 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     8161 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     8221 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     8281 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     8341 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     8401 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8461 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8521 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8581 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8641 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     8701 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8761 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8821 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8881 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8941 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     9001 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     9061 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     9121 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     9181 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     9241 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     9301 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     9361 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     9421 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9481 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9541 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9601 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9661 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     9721 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9781 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9841 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9901 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9961 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10021 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10081 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10141 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10201 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
