LOCUS       sequence-74318-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             94..141
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 atgccacctt ctactagtct tttgttgctt gctgcacttc tccccttcgc tcttcccgct
       61 tcagattgga agacaggaga agtcaccgct tcccgcgacc acatggtgct gcacgaatat
      121 gtgaacgctg ctggcatcac aggcggtggc ggatctggcg gcggtggtag cgtcgacttt
      181 cagaaagcta gctctattgt ttataagaag gagggggaac aggtggagtt cagtttccca
      241 ttggccttca cagtcgagaa gctcacagga tcaggagagt tgtggtggca ggccgaaaga
      301 gcctcctcca gcaagagttg gatcactttt gatctgaaaa acaaagaagt gagcgtgaag
      361 agagtcacac aggaccctaa gctgcagatg ggcaagaagt tgcctttgca cttgacactc
      421 ccccaagccc tgccccagta tgctggctcc gggaacctca ccctcgcctt ggaggctaag
      481 accggaaagt tgcatcagga ggtcaacctc gtggtgatga gggcaacaca gctgcagaag
      541 aacctgacct gcgaagtgtg gggtcccacc tcacctaaac tgatgctgtc tctgaagctg
      601 gagaataagg aggctaaagt cagtaaaaga gagaaggctg tgtgggtcct taacccggag
      661 gcagggatgt ggcagtgtct tcttagcgac tctggacagg tgctgctgga gagcaacatc
      721 aaggtgcttc ctacttggtc aacaccagta cagccgatgg
//
