LOCUS       sequence-74329-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Henry GL, Davis FP, Picard S, Eddy SR
  TITLE     Cell type-specific genomics of Drosophila neurons.
  JOURNAL   Nucleic Acids Res. 2012 Oct;40(19):9691-704. doi: 
            10.1093/nar/gks671. Epub 2012 Aug 1.
  PUBMED    22855560
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks671"; journalName: "Nucleic Acids Res"; date: 
            "2012-10"; volume: "40"; issue: "19"; pages: "9691-704"
COMMENT     Sequence Label: pMUH_unc84_2XGFP
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(195..433)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(227..248)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    464..558
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    592..686
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 gtggcgatct ggcgtttcga actggtggag tcaggagaag cttccggcga acttttgcaa
       61 aaatgtttgg agaattgcgt ccagagcgtg tggatgcgaa ttccgatttc cattcatggg
      121 tgtcgaagtt gttgtcggct tccgttgcgg gagccattct agaggtaccc tcgagccgcg
      181 gccgcagatc tattcagagt tctcttcttg tattcaataa ttacttcttg gcagatttca
      241 gtagttgcag ttgatttact tggttgctgg ttacttttaa ttgattcact ttaacttgca
      301 ctttactgca gattgtttag cttgttcagc tgcgcttgtt tatttgctta gctttcgctt
      361 agcgacgtgt tcactttgct tgtttgaatt gaattgtcgc tccgtagacg aagcgcctct
      421 atttatactc cggcgctcga cgtcgctagc gctagagtct ccgctcggag gacagtactc
      481 cgctcggagg acagtactcc gctcggagga cagtactccg ctcggaggac agtactccgc
      541 tcggaggaca gtactccgac ctgcaggcat gccctagggc tagagtctcc gctcggagga
      601 cagtactccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
      661 tactccgctc ggaggacagt actccgacct gcaggcatgc aagcttgcgg ccctggccac
      721 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
      781 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgca
//
