LOCUS       sequence-74345-e        2832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for transcribing SRA nucl 419-771 of NM_001035235 with T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   McKay DB, Xi L, Barthel KK, Cech TR
  TITLE     Structure and function of steroid receptor RNA activator protein, 
            the proposed partner of SRA noncoding RNA.
  JOURNAL   J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 
            10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30.
  PUBMED    24486609
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2014.01.006"; journalName: "J Mol Biol"; date: 
            "2014-04-17- 17"; volume: "426"; issue: "8"; pages: "1766-85"
COMMENT     Sequence Label: SRAcode_in_pUC57_v3(419-771)
FEATURES             Location/Qualifiers
     source          1..2832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(611..627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(624..646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    635..651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(659..665,666..683,684..689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    704..725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(842..859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1013..1601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1093..1112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1772..2563,2564..2632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2395..2414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2633..2737)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2805..2823
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgta atacgactca ctataggtac ctttggtgcc ttgtgaccag ggccctgatg
      241 gttcattaga tggagccttc gagtcttagg gagttgccgc agggtcccca cagcggctcc
      301 cgacggttgt gaaccagcat ccatcctcca cggattccgg caacccgcct ggccctggac
      361 gtgtctcaac tggcccgcgt gaggggccgc cccggaaatg acgcgctgcc ccgctggcca
      421 agcggaagtg gagatggcgg agctgtacgt gaagccgggc aacaaggaac gcggctggaa
      481 cgacccgccg cagttctcat acgggctgca gacccaggcc ggcggaccca ggcgctcgct
      541 gcttaccaag agggtagccg cagaagagcg tcgactgcag aggcctgcat gcaagcttgg
      601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc ccttccgctt
      841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2761 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2821 ggccctttcg tc
//
