LOCUS       sequence-74346-e        3061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for transcribing SRA nucl 419-999 of NM_001035235 with T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   McKay DB, Xi L, Barthel KK, Cech TR
  TITLE     Structure and function of steroid receptor RNA activator protein, 
            the proposed partner of SRA noncoding RNA.
  JOURNAL   J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 
            10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30.
  PUBMED    24486609
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2014.01.006"; journalName: "J Mol Biol"; date: 
            "2014-04-17- 17"; volume: "426"; issue: "8"; pages: "1766-85"
COMMENT     Sequence Label: SRAcode_in_pUC57_v3(419-999)
FEATURES             Location/Qualifiers
     source          1..3061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(840..856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(840..856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(853..875)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    864..880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(888..894,895..912,913..918))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    933..954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1071..1088)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1242..1830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1322..1341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2001..2792,2793..2861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2624..2643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2862..2966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3034..3052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgta atacgactca ctataggtac ctttggtgcc ttgtgaccag ggccctgatg
      241 gttcattaga tggagccttc gagtcttagg gagttgccgc agggtcccca cagcggctcc
      301 cgacggttgt gaaccagcat ccatcctcca cggattccgg caacccgcct ggccctggac
      361 gtgtctcaac tggcccgcgt gaggggccgc cccggaaatg acgcgctgcc ccgctggcca
      421 agcggaagtg gagatggcgg agctgtacgt gaagccgggc aacaaggaac gcggctggaa
      481 cgacccgccg cagttctcat acgggctgca gacccaggcc ggcggaccca ggcgctcgct
      541 gcttaccaag agggtagccg caccccagga tggatccccc agagtccccg catcagagac
      601 ttctcctggg cctcccccaa tggggcctcc acctccttca agtaaggctc ccaggtcccc
      661 acctgtgggg agtggtcctg cctctggcgt ggagcccaca agtttcccag tcgagtctga
      721 ggctgtgatg gaggatgtgc tgagaccttt ggaacaggca ttggaagact gccgtggcca
      781 cacaaggaag cgaagagcgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg
      841 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      901 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      961 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1021 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc cttccgcttc ctcgctcact
     1081 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1141 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1201 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1261 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1321 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1381 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1441 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1501 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1561 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1621 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1681 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1741 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1801 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1861 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1921 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1981 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2041 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2101 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2161 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2221 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2281 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2341 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2401 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2461 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2521 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2581 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2641 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2701 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2761 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2821 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2881 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2941 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3001 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3061 c
//
