LOCUS       sequence-74359-e        9734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Caenorhabditis elegans of the luc+ gene (Promega), 
            fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid
            pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Lagido C, Pettitt J, Flett A, Glover LA
  TITLE     Bridging the phenotypic gap: real-time assessment of mitochondrial 
            function and metabolism of the nematode Caenorhabditis elegans.
  JOURNAL   BMC Physiol. 2008 Apr 2;8:7. doi: 10.1186/1472-6793-8-7.
  PUBMED    18384668
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Physiol."; date: "2008-04-2"; volume: "8:7. doi"; pages: " 
            10.1186/1472-6793-8-7"
COMMENT     Sequence Label: pSLGCV
FEATURES             Location/Qualifiers
     source          1..9734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3716..5365
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3782..3800)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(5411..5439)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6187..6207
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7174..7196)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7296..7315
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7393..7411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7479..7583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7584..7652,7653..8444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7802..7821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8615..9203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9104..9123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9357..9374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9491..9512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9527..9532,9533..9550,9551..9557)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9565..9581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9570..9592
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9589..9605
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9589..9605
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcattg ctaaattgca accccgtgtt tttgaaaaaa tacatcataa
       61 cagagaaaac tctttaaaat catattgttc agccaggtca cagtcaagga caattttgaa
      121 tttgactcaa tttcagttac aggctcggat aacggcgctg ccacgaccac cctggcccct
      181 tctgacccaa cttgttaaat attaatctaa atacaaataa gatttagtat gaacatttac
      241 tgttcatttt gaatgttgtt tttttacaca ttttcgtagt tgaaaatttt gacatatttt
      301 aagtgttgga aaagatatga gacatttgag cgaactcgtc caaatttgtg aaaatttcca
      361 aaaatcatcg tgaaaaatta aaaaacatat tttcagagaa aaggcttaaa catttttgaa
      421 tatgaataag taaccttttt taaaaatcgg catgtttttt tctttgaaga tcaatatttt
      481 tgcgaaagcc tacgatacta aaaaatatta acagcaaaaa ggtgtaaaat taaaaagtga
      541 ttgtttcatc tgttgacaaa agttttgtca aacaactggt tttggaattt atatgcaaaa
      601 acctgtaaaa agagtgttta ttgaatcttg gacttgactt gtttcatcgg actgtaattt
      661 agcaggggct taagatagga tgaattggta aagtgataaa atatagctat tggtatgcaa
      721 aatattgtta caattgatta ctttttggta tcattcacgt ttctgaagtt accgccactc
      781 aaagttaaat aaaataattt ttgcaagcat ttttgacaac ttaaacgaat atggcgggac
      841 caaaaaggcc atgcctggag ctgttattgc atgtttttga taaacttact gtcatacatc
      901 agggtacata aggtttttga tttcacacaa agaaaaaaaa atcgaaaaaa aaacaatttt
      961 tttccgttgt catagttgaa aaaccctgtt gctaaacaat tcttctaatt tttcgtggta
     1021 actgctacac aatcggatta aggaaaccta attttttctc acatatgttg gagagcctcg
     1081 atattcttga actttttgca ttcattgttt aatgtttctt cacatagctt cgtatatatt
     1141 gccagcaatg caaatcttga gccatcggtt gaaatgattt tgaaaaggtg ctcggcatat
     1201 ctgggctgaa aaaaaacttt tgtttcaacg cattaaacac ttttcggggt tctgttccaa
     1261 ttttttaaat agttgtaaca cccgctagaa tttccgtttc accaaagaaa tctttgttca
     1321 cagattatta tatatttctt ttttatgtat tttgtccagg tgatcctaaa tgtttaaaaa
     1381 acgttccagc agcttttagt ctatacgaag tatatttttt cttattttgg tttatcggag
     1441 gtatgcagtt ccaaatgttg gattttctac cctactagaa atttccttta gttatacaat
     1501 tttaattcga agttgtatct tgaaaaggaa accatcatca acaacaaata agcttgagac
     1561 tagaattgct aatcgtctgc accaaatatg aaattcgttg aataaattat ataaaacagt
     1621 tgacaatcat aaagtatttt acatacatat gtatagttaa tgtcatatta gcatatttag
     1681 tattgagtta acacgcagaa ataccaaaga aaccctgttt aattcatttc catgttttat
     1741 tcgctttaac tagggaatga ccagaataag tggagcgata ttcaaaaaaa aaaaattgta
     1801 tcggaaagct gacattctct actataagaa tatgactgaa atttttgccc attcgggctg
     1861 gaaatctgaa tggaaattgt ttatatgagg ggatccatgg ttgtagttac aattttgaga
     1921 aaaaagttta aattttaggg gaaaagatgt tgaatgaatc attatggtgt tggtcgacac
     1981 attgatcatg ctgatatcga tcagaaaaat taaatttggg cttttatata gtttcgaaaa
     2041 aacacgttta aatttttgaa aaattttacg ttagggtgga attgaacccc agagtcccac
     2101 catgcttgtc atccactcta tccactcggc catctcgctg ttgcaggcag tactaattgg
     2161 ggatagtgaa aagagttaac gtagaatttc agacgtaaaa atttcagatt tccagcccga
     2221 atgggccaaa atttcagtca tattcttata gtagagaatg tcagctttcc gatacaatat
     2281 tttttttttg aatatcgctc cacttattct ggtcattccc tagtaagttt tttcagtttt
     2341 accagaaata aaaccactca taaaaactat aaactacaca tttaaactgt aaatgttgga
     2401 aatcattttg accatcaaac aatagctatc ggaaaatgca aaggccattc cgtgatggca
     2461 cgctcttctt ctatccatcg ctccttttca acgaaacaca cacacaaacg cagactattg
     2521 gaaaagaaaa atatagaaaa aacctttttt ctccttgtcc cttgtgttga tctcactttt
     2581 cagtggacgt gtgcagtgtg gaggccagag actgtgctga ccgacggacg cgcaggcggt
     2641 aaacatacgt tgcagtggag agtgtagtcc ctcgtcggct ctctgtgtat ttctttcaat
     2701 gcctgtgtct cggtctatct ttgtctctat ctgttttaaa agttttttgc aaagtttcaa
     2761 tttctttttt attttctctt ctttttttgc gcatttattt tttttaaaga gcgctgttct
     2821 tcaattggaa aacagtataa cgatcgaaat aaaaaaaaaa cttgaaatag tatcggaatt
     2881 gagatggggg caattgaaat tgaagacgca acaaaaacat gtttttatcg tctgaaatta
     2941 tctaaacttt gaatctaacg tgtcaatggc tacttttaca gtagactttt tagagtaatg
     3001 ttaaaaagga taatataggt ttttcttcaa actattttgt cacgccgcat atattctttt
     3061 catattccat tcgcaaagtg catgatgtaa caaaaatgaa acgtcataat tttttaatct
     3121 ttcacacttt ttttgccggc cgttaaatca aaatcaaatc aacttaatcc aacacgtgcg
     3181 ggcttgcaat tttattacac cggtgtccta cagcgccatg ttatcttctt tgattagaga
     3241 tctctcacgc aacatttgac attgaaaatt ttgtctccga attgttgtgt acctactttg
     3301 tcccgatgtc ccaaattttc actgataaca agagatatct caactgttct tatcacgttg
     3361 atacgtagaa cgttttacac taaaccaaat tgcagtcccc ttctacgaac cgtttgctaa
     3421 ttgttttgca tagaacagtg atcaatgtgt tgtgctcact agtcaatgat gttcgtctct
     3481 catttttcct acaacttcac tgaaaaggtt ttgaatatct ggtgattgta ggcaatcaca
     3541 ccacagttaa tggcctggca aataatgata agcgtgtgat aaacctcaca aaaatacgcc
     3601 tagaagtggt gatatcgcta gttttgtgtc ctttcgattt ttttcgtgtt gcttttttct
     3661 gtggataaac ctttgaactt tacattttgt tttcagaaag cccgggaagc tttccatgga
     3721 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
     3781 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     3841 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
     3901 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     3961 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     4021 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     4081 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     4141 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
     4201 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     4261 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
     4321 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
     4381 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     4441 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     4501 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     4561 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     4621 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     4681 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     4741 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     4801 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     4861 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     4921 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     4981 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     5041 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     5101 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     5161 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     5221 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     5281 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     5341 caagaagggc ggaaagatcg ccgtggtacc ggtagaaaaa atgagtaaag gagaagaact
     5401 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     5461 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     5521 ttgcactact ggaaaactac ctgttccatg ggtaagttta aacatatata tactaactaa
     5581 ccctgattat ttaaattttc agccaacact tgtcactact ttctgttatg gtgttcaatg
     5641 cttctcgaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
     5701 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtaa
     5761 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtg ctgaagtcaa
     5821 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     5881 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     5941 ggcagacaaa caaaagaatg gaatcaaagt tgtaagttta aacatgattt tactaactaa
     6001 ctaatctgat ttaaattttc agaacttcaa aattagacac aacattgaag atggaagcgt
     6061 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     6121 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     6181 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     6241 atacaaatag cattcgtaga attccaactg agcgccggtc gctaccatta ccaacttgtc
     6301 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     6361 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     6421 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     6481 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     6541 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     6601 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     6661 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     6721 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     6781 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     6841 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     6901 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     6961 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     7021 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     7081 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
     7141 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7201 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7261 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     7321 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7381 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7441 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7561 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7621 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7681 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7741 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7801 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7861 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7921 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7981 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8041 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8101 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8161 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8221 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8281 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8341 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8401 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8461 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8521 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8581 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8641 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8701 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8761 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8821 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8881 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8941 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9001 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9061 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9301 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9361 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9421 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9481 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9541 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9601 atgaccatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
     9661 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa
     9721 cttggaaatg aaat
//
