LOCUS       sequence-74365-e        9220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with the S. pombe spo5 gene DSR element into the 
            S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-spo5DSR
FEATURES             Location/Qualifiers
     source          1..9220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1354
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1449..1468)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1568..1590
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1628..1646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1714..1818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1819..1887,1888..2679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2037..2056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2850..3438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3339..3358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3592..3609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3726..3747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3762..3767,3768..3785,3786..3792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3800..3816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3805..3827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3824..3840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3824..3840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3859..3892)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4026..4742
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4026..4739
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4958..4975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4958..4974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7869..8963
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atgtttatga
     1201 gtatacctag tctagaaagg cttgtattta aaatattatt caataaaaat tcacaatttt
     1261 tacgacatgt gctaccattc acttaacttc ctgattataa aattggttcg tttatactaa
     1321 ttacttaagt acctttaact aaacaaaatg cctatatata tattaattta caatgagtgt
     1381 cagataagtc actatgtccg agtggttaag gagttagact cgccatcgat ggtatggtgc
     1441 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     1501 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     1561 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     1621 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1681 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1741 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1801 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1861 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1921 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1981 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2041 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2101 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2161 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2221 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2281 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2341 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2401 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2461 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2521 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2581 cgtatcgtag ttatctacac gacggggagt caggccacta tggatgaacg aaatagacag
     2641 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2701 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2761 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2821 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2881 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2941 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3001 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3061 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3481 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3541 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3601 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3661 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3721 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     3781 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     3841 catgattacg ccaagcttat aacttcgtat agcatacatt atacgaagtt attgctagcc
     3901 gttttcttca attactaaat tagaactaat tcaatcatat gctggcatgc ggagagctcg
     3961 gagtcgacga gactagtact ggttctacag gatcaaccgg ctccacgggg tcgatgggta
     4021 gatctatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag
     4081 atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt
     4141 acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa
     4201 ccttagtcac tactttaact tatggtgttc aatgtttttc tagataccca gatcacatga
     4261 aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt
     4321 ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct
     4381 tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc
     4441 acaaattgga atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga
     4501 atggtatcaa agttaacttc aaaattagac acaacattga agatggttct gttcaattag
     4561 ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc
     4621 attacttatc cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg
     4681 tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa ttgtacaaat
     4741 aaagatctcc cgggactacg ccatatcatg cccatcccgc tacttctgct gctaattaaa
     4801 tttaaacatc tttccagcat ttacttgaac atcaaactct aatatcgaaa tcttttcaaa
     4861 cacttatcac atactcaaac ttaaaccaac ataaaacctg ttagacaaag ccccgggata
     4921 acttcgtata tggtattata tacgaagtta tgaattcact ggccgtcgtt ttacaacgtc
     4981 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5041 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5101 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5161 accgcatacc atcgtccgga tcaatgaagt agatgagttt actacctgta tatacttttt
     5221 tttttgtcaa agtcactttt atggcatttc catgttgaag gattagccac agcactaaat
     5281 tttcagcgta tgattgcttt taaatattta attttcatcg ttttttaatt aatattcaaa
     5341 acgatttaat gccttctaca tctgaagtca agctcataga ctcgggttgg gtagctaaaa
     5401 ctacatctaa cacgaagggg tatagccttc ttaaagtata ctcatgtata aacgttttgg
     5461 gttcatattt ttgggccacg tggtttataa aaattcttaa ctacaccact cggtatcccg
     5521 cacccgtcta cgtttctacg atttcgagct aatattgagt aaagtgatta gcaaaaaaaa
     5581 taaaagtact cgttgtcgga gatcaagaat ttttctatta tctcatctaa accactttct
     5641 aaaagcgaaa aacaaaatcg taatatgcag cttgaatggg cttccatagt ttgaaagaaa
     5701 aaccctagca gtactggcaa gggagacatt ccttttacct ggctaatcgc catcttccag
     5761 caggcgcacc attgcccctg tttcactatc caggttacgg atatagttca tgacaatatt
     5821 tacattggtc cagccaccag cttgcatgat ctccggtatt gaaactccag cgcgggccat
     5881 atctcgcgcg gctccgacac gggcactgtg tccagaccag gccaggtatc tctgaccaga
     5941 gtcatcctta gcgccgtaaa tcaatcgatg agttgcttca aaaatccctt ccagggcgcg
     6001 agttgatagc tggctggtgg cagatggcgc ggcaacacca ttttttctga cccggcaaaa
     6061 caggtagtta ttcggatcat cagctacacc agagacggaa atccatcgct cgaccagttt
     6121 agttaccccc aggctaagtg ccttctctac acctgcggtg ctaaccagcg ttttcgttct
     6181 gccaatatgg attaacattc tcccaccgtc agtacgtgag atatctttaa ccctgatcct
     6241 ggcaatttcg gctatacgta acagggtgtt ataagcaatc cccagaaatg ccagattacg
     6301 tatatcctgg cagcgatcgc tattttccat gagtgaacga acctggtcga aatcagtgcg
     6361 ttcgaacgct agagcctgtt ttgcacgttc accggcatca acgttttctt ttcggatccg
     6421 ccgcataacc agtgaaacag cattgctgtc acttggtcgt ggcagcccgg accgacgatg
     6481 aagcatgttt agctggccca aatgttgctg gatagttttt actgccagac cgcgcgcctg
     6541 aagatataga agataatcgc gaacatcttc aggttctgcg ggaaaccatt tccggttatt
     6601 caacttgcac catgccgccc acgaccggca aacggacaga agcattttcc aggtatgctc
     6661 agaaaacgcc tggcgatccc tgaacatgtc catcaggttc ttgcgaacct catcactcgt
     6721 tgcatcgacc ggtaactttt agattaacat ttgtttaaat atgaaattag gcaatgtaac
     6781 atactgcagg caaattttgg tgtacggtaa gtaaattgga cataagattt aacaaagcga
     6841 ctataagtca gaaagtgaga atgagattga aataattaat tcaacttatt caattgatga
     6901 tatgccagga ttcctcttcc tttatcttca atgtttcgat taacaggtcc attatccggt
     6961 ttttagtttc gctgcacatt gccgaatgac aatcggattc ctttaagcta tgcttttgcc
     7021 ctgttttctc tccccagtaa tcgttgggtg actgaaccat ccagtagccg agagttacgt
     7081 ttttcctgat ttaataaata tccatatcta tttgtctcgt ggtgttttac agcaactaac
     7141 cgaaattact attttgcatc atccaaccat aaagagacaa caataattct agaaaacata
     7201 taacaagtgt ttttttctat ttacgtctca tacgcaaagt ccactttacc atgttttgga
     7261 aacttgtacc caaccattca tttttcgatt ttgtaatgag gctcttttct tctaacaatc
     7321 attgaggtca ttatcatgtg tcctcaccta ttgtttaaat tgaggcttgc gctattccca
     7381 aaattgtggg tattattttc atatatcagt atctgcaatt tcggttcaac tccggaagct
     7441 cggtacccaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
     7501 aaccattatt ttttttctct caacataacg agaacacaca ggggcgctat cgcacagaat
     7561 caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt
     7621 ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat
     7681 agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt
     7741 atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt
     7801 tcttaccttt tacatttcag caatatatat atatatatat atatatattt caaggatata
     7861 ccattgtaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag
     7921 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca
     7981 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac
     8041 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg
     8101 gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta
     8161 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag
     8221 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gttagagaat
     8281 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg
     8341 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg
     8401 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct
     8461 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgaattt cctacattga
     8521 aagttcaaca tcaattgatt gattctgccg ccatgatcct agttaagaac ccaacccacc
     8581 taaatggtat tataatcacc agcaacatgt ttggtgatat catctccgat gaagcctccg
     8641 ttatcccagg ctccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga
     8701 acaccgcatt tggtttgtac gaaccatgcc acggttccgc tccagatttg ccaaagaata
     8761 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact
     8821 tgcctgaaga aggtaaagcc attgaagatg cagttaaaaa ggttttggat gcaggtatca
     8881 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag
     8941 aagttaagaa aatccttgct taaaaagatt ctcttttttt gtgatatttg tacataaact
     9001 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg
     9061 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg
     9121 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
     9181 gcactttaac attaatattg acaaggagga gggcatcaca
//
