LOCUS       sequence-7437-ex        9967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, 
            Weinstein BM
  TITLE     Notch signaling is required for arterial-venous differentiation 
            during embryonic vascular development.
  JOURNAL   Development. 2001 Oct . 128(19):3675-83.
  PUBMED    11585794
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2001 Oct . 128(19):3675-83."
COMMENT     Sequence Label: 41 pBS119 d10L
FEATURES             Location/Qualifiers
     source          1..9967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5423..5434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9282..9298)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9283..9299)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9323..9342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9324..9342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9352..9369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9352..9368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9361..9383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9510..9965
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9597..9616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9807..9828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag agcgtgctcg gtaactgaaa aagagggggt ggagattttg gatttagaga
     2281 tggaatcaca tgcacgctgt atatgctata taaaaatagc aggcatttaa tgcatgcaaa
     2341 atatgtttcg acactaaaca ctacaagtcg gaaatatatc tatttttctt tttttaacga
     2401 aatgagacat ttaatgcgca gtaaatgtag tctaacacgc acaatattcc tattattgtc
     2461 catgaaaaat gtgaggtcnt atgctactga tttaaagaaa atgttgaaca ggcacagcac
     2521 acaagaggta tcagcctctc tatatggtcc atacagcacc aggctcgctg cgttcatgtg
     2581 cattcagagg cgcgcacacg tgtggagcag acagactcga cgcagcgggc tttgcttttt
     2641 attcatggaa gcccatttta gaagaagctc gggcagactc gtcacattct tcacttcacc
     2701 ataggtagat caaatcaaag ttattggata taancggaac aatattcatc atattatgna
     2761 gattattatt aagaccgagt gcattagaca tattttgtat ttcgatcgtt ggaaaaagta
     2821 tatattttat tttagcctta aactatggtt agcctatatt aatcagcata atctgttatt
     2881 tcagacgttg tattaatgat tatttctagc acagctttga tgcatcaatg caatgtttct
     2941 acaaacccgc cttgtttaat gtcggaaaaa aagcgcaaca aatgtaaaga gttggcaggc
     3001 attctgtaaa tatttccagg caaaaacatg cacaaaggaa agaaacacca ttgttttggt
     3061 gtcttatctt cgctggacaa atatcctgct ttgggtgccg taatgaaggc agggccagtg
     3121 acacacacac atacacacac acacatacac gcatacacgg cttccttctc aagcgcagtg
     3181 acttcacttc ccaaatttag aggaaaaaaa accatccggc gaaactgtct ctgtctctcc
     3241 gccacatatc gggggctgga attaattcag acgcgctagc ccgggcggcg cgccgcgtgt
     3301 catgtttgga atattgtcgg gctccactga aaattgcggc aaaatggacg gaactattaa
     3361 ggtaagagtg gaggagaagg gatctttcgg aagcatggac ggagttcgag agcgtagacg
     3421 cctgtatttc gggctgtctc agccgctccg agctgctctt ttcctcttct ccctctctct
     3481 ttctcaagtt tccttttcct tttcgtcgaa actttgttga aagttgaaag ctatagcgga
     3541 ggatacgcgc gttcagacgc acgctcgtaa aactaacggg ctagactcgc tgttgtgatg
     3601 gagcaaagag ttgagggaaa atccgctttg tttttaagat gaaaacagcg aatgtcttct
     3661 agtcgacgag actgcttgct ttgggttttc agcatatcgt ggcgcgcgcg tgtgtgtgtg
     3721 tatgtgtgtg tgaatgcagt gcagtggttt gtataaatca taaagcttta ggctggagat
     3781 gtggctgcgc acttggagtg gcaccgtgcc gaggttttcc aaccattcat atagttcaaa
     3841 agcggagccc acccacaata aaatatcact ccacagtctt tgccgcattc tgataactca
     3901 aaataattgc tataagctaa tcttaattgg gctgtgatat cgcaaattag gctgcttaag
     3961 aaaaaaaaaa acgcggtctg cttagaataa ttagctatgt gtggtcctca cattttcaat
     4021 ctccaaatag ctattttaac atgtgcataa ttattaaatc catgtctgaa aagacaccag
     4081 tagctatttg aattaaatat tggtgagcgt catatttaag gtgtaatgat ctctgattaa
     4141 ttgttggctc ctgtctggag gggagggcta caaatgcaaa cactcctttt tttttgcggt
     4201 catataagtg gttttgggtg tggtatatgt ttggggccgt acacatcatg cttgtgttag
     4261 attttatcat gttaaattgg cttttttcga tagcaatggg gtgactgagg acagaaaatg
     4321 gtattttttg gcgcataaag ggcaaaagat aactgttgtg aaatatataa cattaggaat
     4381 tggaatattc tgtttgtttt tatataatct gtgttgtcat tgctagataa aatagtttat
     4441 taacctaaaa aaatgttttc ttttattttt ttgtttgttc aaacttaaaa atggaatatc
     4501 aattagtcct gacagcagat gtttttaggt cattgtaact tattaaacca agtaattcat
     4561 gttctaactt aatttttttt aagttacgca agctgtttta agtcagttta acataatata
     4621 tgttcaatga actcataaag ttaatttgat tcagctttaa aaaatatgca accaggattt
     4681 tttttgcagt gctnatttat aatgattatt attattatta ttattattat tattattatt
     4741 attataatta ttattatcat tattatttta agatgccaat gtaaaattta cctaattgta
     4801 gctattatag aatgtcagtt ttttaatgta aataaatcaa tcataatcat aaataaactc
     4861 aatttaaata tgatttacaa aaaaaaatcc tttaaaatgt aatttagtgt aacaatagca
     4921 agtaaacaca ttgattaata cataacaatt aaacattgat tgattcataa ttagaataat
     4981 aattgtttga ttacattaaa agcgcttaat ttggccacaa catattaatt taatgtcatt
     5041 tgaaatatta agagttttgt catcgccact attttttaag ctatttgtca gcaggaagtt
     5101 tttaataaat aaaatagtat aaaatctagt attataaaat attaaatgtc aagtttctat
     5161 ctaagttcag tgtacatgga atattacatt aacaccaata aattgatatt ttattttcac
     5221 aaattttgaa atttgaaaca agtatacctt tcctttaatt taactgatat aaaaatgtat
     5281 gatcatatta tttttcatgt tcatataaat atatatatga tcttgatgaa gacggtaaat
     5341 tatactatca accatatttt gatatattgt cacaaaaaca tttaaaaatg gacaagtaga
     5401 ctctttactt taagttaaca tgccttcctt ctatacaatt catttattga cagtaaggac
     5461 accaaaccag gagatctcat ctttgaccca taaacataca ctaaaaccca acagtcaact
     5521 ttatcaaatg aaatgagtgt aattaactca aaatttactg aatgttaatt ctactcattc
     5581 gaaaagagtt ttaaactcag tgttgaaggt agtgagttca ttaaatacct cattacttca
     5641 acttaactgg agtaagttca tggtactcac atagattagt ttagctcaaa tggtttgtag
     5701 cagtcggttc cctcaagcag tttgagttgc cttaactttt tgggttttac agtactcagt
     5761 tggtttgagt tcacttcact tattgggttc aacatgtgct caaatgcatc gttttaatca
     5821 aatagattaa gtttcacagt tactcatttg gattagtttt tgaacttaaa tggtttgttg
     5881 cagtcggttt cctcaaacgg tttgagtttc cataacttta tgggtttttc agtgtagtaa
     5941 acatatgcat ccacagatta taatagtaac ctgcatgttt tttccttcac aatttgacac
     6001 agatacctca catgattgac ttttaactgt acactttagt cgacaatgag aagggtgtct
     6061 caatctatgg gtatttcctg ttccacacaa tctggtttcc tgtcaggttt ccttttcctc
     6121 ctgaaacaaa agacaggaat gccctctgta taaaatagca cagcgctttt aatgggtctc
     6181 tgtacatccc gacctcatga cgcatcttct gcgggacagc tcaatcacga tcaagttggc
     6241 ttatgaaaac agttttttgt ccccctgtta agttaaaagc acggtagcat acatgcatta
     6301 ttcatcatgc agcatattca gcagtacaaa agatgagctc ttgacacacc tcagcgtaaa
     6361 atctctattc cagcgtaatc ctgcggttct ggatggttaa gcgagccttg caaacacggc
     6421 caatttatag ccatgttgaa aatagcagct ttgtgagcat ttcgacctca atgtaccaag
     6481 aatctgcact tactgtaaaa aatagccaca aattagcagc ttttcatatc ttgtgattca
     6541 tgtttttatt ttattttgat cttatttctg cttttaaatt gcattattgg atcttgagct
     6601 ttcttccaac aacttttaac cttgaaaagc gtgaaaaagg gacttttatg aacattttta
     6661 atattttaaa gtagggctgc acaatatatc gtgttgagca tcgatatcac aagctgtgca
     6721 cacacaatag tgtctttaat tggcattatt tatatagtta agaaatatat aatgttgaag
     6781 tcagaattat tcgcccccct gtttattttt ccccaatttc tgtttaacgg aaagaagatt
     6841 tttctttata aaaataatac ttttaatagc tcatttctaa taactggttt attttatatt
     6901 tgtcatgatg gcagtaaata atatttaact tgatattttt caagacactt ctatacagct
     6961 taaagtcaca tttaaaggct taactaggtt aattaggtga actaggcagg ttagggtaat
     7021 taggcaagtc attgtataat gatgggttgt tctgtagact atcgaaacaa aaattagctt
     7081 aaaggggcgt aataattttg tccttaaaat tctttaaaaa aaattaaaaa ctgcttttat
     7141 tctagccgaa ataaaacaaa taagacttct ccagaagaaa aatatattat cagacatact
     7201 gtgacaatgt ccttgctctg ttaaacataa tttaggaaat atttcaaaac gaagaaaaaa
     7261 attcaaaggg ggtctaataa ttctgacttc aactatacat tgctcagcat atataagtac
     7321 acccctcaca aatctatatt ttaaagtcat atttttaata ggagagcaat tccggcatta
     7381 tgaatgtgac atttacagta aaagctcaaa acataaattc acatagaacg tatacgtcag
     7441 cattaaatta aggcaactgt ttatgttctc caaaacaact aactactaca gagttatggg
     7501 atcagaaaaa tatcaatctg taaacatttt ctatatttta aatgaggaaa ataaacatga
     7561 gttatggatg tgaccaaaaa acatacaaca tacttgatag aaagtctgtg aattaaactg
     7621 cactagccaa aagaaataac gctcatcaaa aggagaggag ttcctattta tacactctca
     7681 gaaataaagg tacaagggct gtcacttggc tgtcactttt caaaagttgc atacagtatt
     7741 tgactttacc taaagtcttt agtgcactct cagaagaaaa ggtacacagt ggtacaaata
     7801 atgttagttg aaggaacagt tttgtccctt tgtaaagatt gtatcttatt caccgtattc
     7861 tccgccgacg agtgggcgct gccatttgaa tctttttggc ttgagacttc cggtctcatt
     7921 cacttccatt cattttttga cgttaaaaac tgctcatttc gctgctcgat gttgcaattt
     7981 gatattttct tattatatta ttctacttgg tctgtgtagt catgcaaaca tttgtttgta
     8041 gagcaggtag tttgaccgtt ttctgccgtt tattattcct agtcatttct cccataggcg
     8101 actgaatcgg aagtcctaag acaatcacga aaacaggcgc acttactgta cgcattttag
     8161 aataaggtca atatggtgca gaaaagacct cttatgatac tattcaatac cttttatggt
     8221 acaactgaaa tgaaggtaca caattgttcc catataaatg gtgcaatagt gttgatcaac
     8281 gttttattta ttacaacacc tatgaaaata aatatgacta gaaaggaaaa gtgattgtat
     8341 gtataatttc taaaagaaag aaaccagtaa ttattaaatt tcataatgca atattacaaa
     8401 acaactggta aaacatatac cataggtatt gtaaactaaa cattaaaggc attttaaata
     8461 aatgtttagt gagcattttt gacactgtta ttgtacaaag tgtcttgtca ctgtggtgct
     8521 acctttatgg atcagatttg tacctttagt gaaggtacga ttttgtatct gtaggtttta
     8581 aaaatcaggt acatttggtt tcccatggta tgatatcctt aaaggtaaaa ctgcaatggt
     8641 acaaatgtgt ttctttgtct taaggtacaa ttttgccccc atacaaaggt actgccccag
     8701 tgacaagtgt ttgtagcttc tttgattaaa cattgtacca ttttttctga gagtgtggta
     8761 cctcaaaggt acatatcagt acccaaatgc nnntaaaatg tacctttgag gtactaatat
     8821 ggactctntc gggtacaatt aaataccttt agaaaaggta ctgccccaga gacagccctg
     8881 tacctttatt tctgagagtg tagaacaggg gtcaccaatc tcagtcctgg agggccggtg
     8941 tccctgcagg gtttagctcc aacttgcctc aacacacctg cctggatgtt caagtatacc
     9001 tagtaagacc ttgattagct tgttcaggat tgtttgatta gagttggagc taaactgtgt
     9061 tggacaccgg ccctcaagga acaagtttgg tgacccctgg tgtaaatcaa aaatcattga
     9121 ttttattaat ttgacatagt ggccaaacta tgaactttta tagtcatcaa acagttattt
     9181 ttctaattat ggggtgttga tctctgctct gatgtctttt gatattgaaa attaaagtct
     9241 agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag
     9301 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     9361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9481 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     9541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9661 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9721 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9841 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9901 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9961 aatttag
//
