LOCUS       sequence-74372-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JxON-post.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-Jx-rev-post-mGRASP-2A-dTomato
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1..33)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(758..811)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 taacttcgta tagcatacat tatacgaacg gtaaagctta cttatacagc tcatccatgc
       61 catacaagaa caggtgatgc cgaccttctg agcgttcgta ctgctcgacg attgtgtagt
      121 cctcgttgtg actcgtgatg tccaacttgg tgtccacgta gtagtagcca ggcagctgaa
      181 ccggcttttt ggccatatat atagttttga actcaaccag gtagtgcccg ccatctttga
      241 gtttaagcgc ctgatggatt tcgcctttaa gaacgccatc gcgggggtac agtcgttctg
      301 tagaggcctc ccaccccatg gtcttcttct gcatcacggg gccatcgggg ggaaaatttg
      361 ttccgcgcat cttcaccttg taaatcaggg tgccgtcctg cagagaggag tcttgcgtga
      421 cagtgaccag cccaccatcc tcaaaattca ttactcgttc ccacttaaat ccttcaggaa
      481 atgacagttt tttgtaatca ggtatatcag cagggtgttt gacgtaagcc ttgctaccgt
      541 acatgaattg agggctgagg atgtcccagg cgaaaggaag ggggccacct ttggtaacct
      601 tgagcttggc ggtttgtgtg ccctcatatg gtcttccttc accctcacct tcaatttcaa
      661 actcgtgccc gttcatgcta ccctccattc taactttgaa tctcataaac tccttaatta
      721 cttcctcacc cttagacacc attgtcgacc ccggggccgg tccgggattc tcctcgacgt
      781 cgccacatgt gagcagagag ccccgaccct cagatctgcc accgctgcca cccacccgtg
      841 ttgtgctgtg ggagtggggg tgtggatgcg gatgaggga
//
