LOCUS       sequence-74376-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(150..203)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttgtcc tcctcgccct tgctcacgac tttcctcttc
      121 ttcttgggca ccatggtcga ccccggggcg ggaccaggat tctcttctac gtcgccgcat
      181 gtcagcagac taccccgtcc ttcgaattcc ttgtacagct catccatacc cagggttatg
      241 ccggcagctg tgacaaactc cagcagtacc atatgatctc tcttttcgtt ggggtccttg
      301 ctcagcttgc tctgtgtact aaggtagtga ttgtcgggca acagcacagg tccatcaccg
      361 atgggagtat tctgttggta atggtcagcc agctgaacgc ttccatcctc tatgttgtgc
      421 cggattttga aattggcctt tatgccattt ttctgcttgt cggcggttat gtaaacatta
      481 tcagatatgg cgttgtattc cagcttgtgg ccgagaatgt tcccatcttc tttgaagtcg
      541 attcccttga gttcaatcct gttcaccaag gtgtcacctt cgaatttcac ctccgccctt
      601 gtcttgtagt taccatcgtc cttgaaaaat atcgtccttt cctgaacata cccctcgggc
      661 atagcggact tgaagaagtc gtgctgcttc atgtggtcag ggtaccgggc gaagcactgt
      721 acaccccatg tgagggtggt gacgagtgtg ggccaaggca cggggagctt ccctgttgta
      781 cagatgaact tcagcgtcag tttgccgtag gtggcatccc cttctccctc gccgctta
//
