LOCUS       sequence-74400-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH83999
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      979..988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccactct gtgaagacaa
       61 cagatgagac gccagtggta tggggtaccg cacggtctgc caatccgacg gaggtttatg
      121 aattttccac cttttccaca agctcaacta gtattaacga tgtggattta gcaaaaaaaa
      181 cctgtaaccc taaatgtaaa ataacgggta agcctgccaa ccatgttatg gcagattaag
      241 cgtctttttg aagggcaccg catctttcgc gttgccgtaa atgtatccgt ttagcaaaaa
      301 ggattaaatt aatgagcacg tattctgaca tttgcatcgt tggcgccggc ataggaggct
      361 tgacttgcgc caacaacctg atcgacgccg ccgccggcag gaacctgcgc atccgcgtat
      421 tcgacctgaa tgccaccgta ggcggccgca tccagtcgcg gaaaatagat ggcgaggaaa
      481 tcgccgaact cggcgccgcc cgctactcgc cgcagctgca tccgcatttc cagcaactca
      541 tgcagggcag cggcttgccg catgcggtct acccgttcac cgaggtcatc tcccacgata
      601 gcgtgctgga ggagctgaag gcaacgctgg atgagctgag cccgatgctg aaaatgcatc
      661 cgaacgactc cttcctcgag ttcgtcagcc attacctggg cgccgccaag gccagccaca
      721 tcatcaaggc gaccggctat gacgccctgc tgctgccgat ggtgtcggcg gccatggcct
      781 acgacatcat caagaagcac ccggaaacgc agcactttac ggaaaacgcc gccaaccagt
      841 ggcactacgc caccgacggc taccacgaat tgctgtgcca gttgcagcac caggcccagg
      901 tcgccggggt ggaattcagg ctcgaacacc gcttgctgtc cgttgaaaaa tcgggcgccg
      961 accatgtgct cgccttcagc caccatggcg ac
//
