LOCUS       sequence-74407-e        2115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based, Golgi-targeted kinase activity reporter for PKD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Kunkel MT, Newton AC
  TITLE     Calcium transduces plasma membrane receptor signals to produce 
            diacylglycerol at Golgi membranes.
  JOURNAL   J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 
            10.1074/jbc.C110.123133. Epub 2010 Jun 2.
  PUBMED    20519514
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.C110.123133"; journalName: "J Biol Chem"; date: 
            "2010-07-23- 23"; volume: "285"; issue: "30"; pages: "22748-52"
COMMENT     Sequence Label: Golgi-DKAR
FEATURES             Location/Qualifiers
     source          1..2115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(136..138,139..849)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(1399..1401,1402..2115)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atgggcaact tgaagagcgt ggcccaggag cctgggccac cctgcggact tggactgggt
       61 ctgggacttg gattgtgcgg caagcagggc ccagccaccc cggcaggtgg aggtggtgga
      121 ggaggtggag gtggtgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      181 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      241 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      301 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      421 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      541 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      601 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
      661 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      721 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
      781 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
      841 ctgtacaagg ctggtaccaa gggtaatggt aggtttttaa ctttaaaacc attgcctgac
      901 agcattattc aagaaagcct ggagattcag caaggtgtga atccattttt cattggtaga
      961 tccgaggatt gcaattgtaa aattgaagac agtaggttgt ctcgagttca ttgcttcatt
     1021 ttcaaaaaga ggcatgctgt aggcaaaagc atgtatgaat ctccggcaca aggtttagat
     1081 gatatttggt attgccacac cggaactaac gtgagctatt taaataataa ccgcatgata
     1141 cagggtacga aattcctttt acaagacgga gatgaaatca agatcatttg ggataaaaac
     1201 aataaatttg tcattggctt taaagtggaa attaacgata ctacaggtct gtttaacgag
     1261 ggattaggta tgttacaaga acaaagagta gtacttaagc aaacagccga agaaaaagat
     1321 ttggtgaaaa agttaggagg atccggaggt ctgtcgaggc aactgactgc agctgtctcc
     1381 gaaggtggat ctggtggagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1441 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1501 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1561 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt cgcccgctac
     1621 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1681 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1741 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1801 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1861 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1921 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1981 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag
     2041 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac
     2101 gagctgtaca agtaa
//
