LOCUS       sequence-74531-e        2307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein 
            engineered to bind a site in the human beta-globin (HBB) gene and 
            demethylate CpGs adjacent to the binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai 
            SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson 
            MF, Joung JK
  TITLE     Targeted DNA demethylation and activation of endogenous genes using 
            programmable TALE-TET1 fusion proteins.
  JOURNAL   Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726.
  PUBMED    24108092
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726."
COMMENT     Sequence Label: JA1266
FEATURES             Location/Qualifiers
     source          1..2307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
ORIGIN
        1 atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat caagcctaag
       61 gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt cactcatgcg
      121 catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt caaataccaa
      181 gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag
      241 tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct tagggggcct
      301 ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg agtaacagcg
      361 gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccttgaa cctgacccca
      421 gaccaggtag tcgcaatcgc gtcacatgac gggggaaagc aagccctgga aaccgtgcaa
      481 aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt
      541 gcaagcaaca tcggtggcaa acaggctctt gagacggttc agagacttct cccagttctc
      601 tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtcgaa cattggaggg
      661 aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg
      721 acgcctgcac aagtggtcgc catcgcctcc aatattggcg gtaagcaggc gctggaaaca
      781 gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc
      841 gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg
      901 gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaacatc
      961 ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac
     1021 gggctgactc ccgatcaagt tgtagcgatt gcgaataaca atggagggaa acaagcattg
     1081 gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa
     1141 gtggtcgcca tcgcctccaa tattggcggt aagcaggcgc tggaaacagt acagcgcctg
     1201 ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgtca
     1261 catgacgggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa
     1321 gaccacggcc ttacaccgga gcaagtcgtg gccattgcaa gcaacatcgg tggcaaacag
     1381 gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc
     1441 gatcaagttg tagcgattgc gtcgcatgac ggagggaaac aagcattgga gactgtccaa
     1501 cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc
     1561 gccagccatg atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg
     1621 tgccaggatc atggactgac cccagaccag gtagtcgcaa tcgcgtcgaa cattggggga
     1681 aagcaagccc tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt
     1741 acaccggagc aagtcgtggc cattgcaagc aatgggggtg gcaaacaggc tcttgagacg
     1801 gttcagagac ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta
     1861 gcgattgcga ataacaatgg agggaaacaa gcattggaga ctgtccaacg gctccttccc
     1921 gtgttgtgtc aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc caacaacaac
     1981 ggcggtaagc aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat
     2041 ggactgaccc cagaccaggt agtcgcaatc gcgtcaaacg gagggggaaa gcaagccctg
     2101 gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa
     2161 gtcgtggcca ttgcaaataa taacggtggc aaacaggctc ttgagacggt tcagagactt
     2221 ctcccagttc tctgtcaagc ccacgggctg acacccgaac aggtggtcgc cattgcttcc
     2281 cacgacggag gacggccagc cttggag
//
