LOCUS       sequence-74569-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS4.2 (FF575)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             384..689
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     568..587
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(730..854)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg agccggctta
       61 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
      121 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
      181 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
      241 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
      301 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
      361 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
      421 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggc cgacggatgg
      481 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
      541 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
      601 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
      661 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
      721 cacctcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
      781 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
      841 ttgtacaaag tggtgatcct agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa
      901 gttcaatgca tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg
      961 cattgggaaa actgtttttc ttgtaccatt tgttgtgc
//
