LOCUS       sequence-74571-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister 
            R, Schulze E
  TITLE     SnAvi--a new tandem tag for high-affinity protein-complex 
            purification.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e91. doi: 10.1093/nar/gkp1178. 
            Epub 2010 Jan 4.
  PUBMED    20047968
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6):e91. doi"; pages: " 
            10.1093/nar/gkp1178. Epub 2010 Jan 4"
COMMENT     Sequence Label: pBY2807
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(82..88,89..106,107..112))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(137..261)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(294..308)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(315..338)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     promoter        complement(362..471)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    487..511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(511..530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(512..530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(572..591)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(653..675)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
       61 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
      121 tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
      181 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      241 agcttttttg tacaaacttg ttgtcatacc ggtggatccc gggcccgcgg taccttgtcg
      301 tcgtcatctg cacccttttc gaactgcggg tggctccagc ttgccatggt atatctcctt
      361 tgattgtaaa taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga
      421 taataattct tatttaacta taatatattg tgttgggttg aattaaaggt cccggattaa
      481 ctccggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcggtcc
      541 gatccaattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct
      601 gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac
      661 gccagaagcc gaaccccgcc ctgccccgtc cc
//
